在癌症和非瘤组织中PAX8表达:在149个不同的瘤实体中的17000多个瘤上进行了一项组织微阵列研究
Natalia Gorbokon1, Sarah Baltruschat1, Maximilian Lennartz1
1Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Virchows Archiv : an international journal of pathology
|August 6, 2024
概括
PAX8免疫组织化学是诊断各种癌症,特别是甲状腺,妇科和脏瘤的宝贵工具. 将PAX8与CDH16结合起来,提高了识别细胞癌的特异性.
科学领域:
- 病理学 病理学 病理学
- 在瘤学瘤学.
- 免疫组织化学 免疫组织化学
背景情况:
- PAX8对于甲状腺,脏和穆勒尔管道发育至关重要.
- PAX8免疫组织化学有助于识别脏和卵巢瘤,但其在其他癌症中的表达受到争议.
- 相互矛盾的数据需要对各种正常和瘤组织的PAX8表达进行全面评估.
研究的目的:
- 综合评估PAX8蛋白表达在广泛的正常和瘤组织使用免疫组织化学.
- 为了比较PAX8与cadherin 16 (CDH16) 在识别瘤起源,特别是脏瘤的诊断效用.
- 评估PAX8表达,瘤等级和特定癌症类型患者结果之间的相关性.
主要方法:
- 使用PAX8免疫组织化学分析大型组织微阵列 (17,386个样本,149种瘤类型,76种正常组织类型).
- 将PAX8表达数据与现有的CDH16数据进行比较.
- 统计分析以将PAX8表达与瘤等级和患者结果相关联.
主要成果:
- 在40种瘤类型中检测到PAX8,在甲状腺,妇科和瘤中呈高阳性.
- 在乳腺,肝脏,胃,前列腺,胰腺和肺腺癌,淋巴瘤和小细胞癌中,PAX8在很大程度上缺席.
- 对于脏瘤,PAX8显示出高灵敏度 (88.1%) 和特异性 (87.2%);将其与CDH16结合起来,特异性增加到96.8%.
结论:
- PAX8免疫组织化学是各种癌症的可靠诊断标记物.
- PAX8表达模式可以帮助区分瘤起源.
- PAX8和CDH16的联合使用为诊断细胞癌提供了高特异性.
相关概念视频
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...