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基于PCR的海洋无脊椎动物标本的DNA条形编码具有成本效益,使用NovaSeq amplicon测序

Genki Kobayashi^1,2, Hirokazu Abe³

¹Research Center for Creative Partnerships, Ishinomaki Senshu University, Ishinomaki, Miyagi, Japan. genkikobayashi5884@gmail.com.

Molecular biology reports

|August 6, 2024

概括

一种新的,具有成本效益的DNA条形编码方法使用下一代测序有效地测序海洋无脊椎动物的基因. 这种方法有助于评估生物多样性,并识别潜在的入侵物种.

科学领域:

海洋生物学海洋生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

人们对海洋环境的高生物多样性知之甚少.
在环境变化导致不可逆转的损失之前,积累核酸序列数据至关重要.
介绍了一种具有成本效益,无图书馆准备的DNA条形码编码方法.

研究的目的:

为海洋生物开发和验证一种可访问的DNA条形码方法.
为了生成海洋无脊椎动物的核酸序列数据,专注于类动物.
利用下一代测序来有效地获取数据.

主要方法:

使用了短目标的多重PCR和索引PCR.
使用了新设计的原始原料与适配器和索引,用于NovaSeq测序.
在95个标本中对四个线粒体和三个核基因进行了有针对性的测序.

主要成果:

在六个位点中,平均每样本2816bp的序列被有效地获得.
与线粒体基因相比,核基因的成功组装率更高.
通过将序列与地理距离较远的标本进行比较,确定了潜在的入侵物种.

结论:

下一代测序与桑格测序相比,提供了高效的基因测序.
该方法是进一步分析样本选择的初步步骤.
需要进一步优化,以提高特定基因位置的成功率.

关键词:

这里是Annelida.潮间区域是潮间区域.超标标的编码是metabarcoding 多重复合PCR是一种多重复合PCR.这是下一代测序.多种类型的多叶植物.

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