在妊娠蛋白限制下下的发育:细胞和分子机制
Bruno Calsa1, Luan Dos Santos Menezes2, José Guilherme Neves2
1Fetal Programming and Hydroelectrolyte Metabolism Laboratory, Department of Internal Medicine, FCM, Campinas State University (UNICAMP), Campinas, SP, Brazil.
Journal of molecular histology
|August 6, 2024
概括
怀孕期间母亲的蛋白质限制会影响胎儿的面骨发育,改变矿物质含量并延迟下矿化. 这项研究揭示了健康和疾病 (DOHaD) 的发育起源.
科学领域:
- 发展生物学 发展生物学
- 头骨面部的发展
- 营养科学 营养科学
背景情况:
- 孕产妇营养不良对面骨发育的影响尚不清楚.
- 孕期蛋白质限制是一个重要的营养挑战,可能会对发育产生后果.
研究的目的:
- 为了研究妊娠期蛋白质限制对小鼠胚胎中骨和下骨质发生的影响.
- 阐明由于母体蛋白质缺乏而导致面骨发育变化的分子和细胞机制.
主要方法:
- 在怀孕期间,小鼠被养正常蛋白质 (NP) 或低蛋白质 (LP) 饮食.
- 在妊娠第18天 (GD18) 的胚胎使用微计算机断层扫描 (μCT),扫描电子显微镜 (SEM),能量分散X射线光谱 (EDS) 和PCR进行了分析.
- 对胎儿介质细胞进行了自动态分析.
主要成果:
- LP后代的体重减少,但头部体积没有显著差异.
- 经过EDS检测,LP胎儿的下和中和的百分比发生了变化.
- SEM表明卡尔瓦里亚上增加了酸沉积,但延迟了矿化和LP胎儿下中的更高的原,以及减少了自和差异性基因表达 (Akt1,Mtor,Nfkb,Smad1).
结论:
- 孕期蛋白质限制似乎改变了子宫骨分化,导致面骨发育变化.
- 研究结果表明,由于母亲的蛋白质缺乏,下巴矿化延迟和自改变.
- 结果有助于理解关于面结构的健康和疾病的发育起源 (DOHaD).
关键词:
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