在罗马尼亚的HTLV-1的南非起源
Olivier Cassar1, Ambroise Marçais2, Olivier Hermine2
1Institut Pasteur, Université Paris Cité, CNRS UMR 3569, Unité d'Épidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Paris, France.
PLoS neglected tropical diseases
|August 6, 2024
概括
罗马尼亚的人类T淋巴细胞病毒1型 (HTLV-1) 可能起源于南非. 遗传分析表明,在罗马尼亚人口中最近引入和传播.
科学领域:
- 病毒学 病毒学
- 遗传学 遗传学 是一个
- 流行病学 流行病学
背景情况:
- 欧洲的人类T淋巴细胞病毒1型 (HTLV-1) 感染通常与来自西印度群岛,撒哈拉以南非洲和南美洲等特有地区的移民有关.
- 罗马尼亚是欧洲唯一的HTLV-1流行病重点,有成年T细胞白血病 (ATL) 的确诊病例.
- 了解罗马尼亚的HTLV-1遗传多样性对于阐明这种特定的特有菌群的起源至关重要.
研究的目的:
- 调查罗马尼亚人口中HTLV-1菌株的遗传多样性.
- 确定罗马尼亚HTLV-1特有焦点的可能地理来源.
- 评估HTLV-1在罗马尼亚引入的近期情况.
主要方法:
- 从外围血液单核细胞 (PBMC) 或11名HTLV-1感染的罗马尼亚人 (9名ATL病例,2名无症状携带者) 的布菲外套中获得的DNA.
- 测序了所有标本的长终端重复 (LTR) 区域.
- 使用多个PCR反应生成了10个标本的完整HTLV-1基因组序列.
- 使用LTR和对原型HTLV-1菌株的完整基因组序列进行了家族遗传学分析.
主要成果:
- 大多数罗马尼亚HTLV-1 LTR序列表现出低核酸多样性 (0-0.8%) 并与之前识别的罗马尼亚菌株RKI2.2密切相关.
- 遗传学分析将所有11个序列都置于HTLV-1a世界性的基因型中.
- 其中10个序列聚集在莫桑比克-南非A子组内,表明它们有共同的起源.
- 一个菌株ROU7与主要集群略有差异 (1.5%).
结论:
- 在罗马尼亚流传的HTLV-1菌株很可能起源于南非.
- 罗马尼亚HTLV-1菌株之间高度的遗传相关性表明,最近的引入事件发生在人群中.
- 需要进一步的研究来确定中欧的HTLV-1聚焦的确切引入路线和时间.
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