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通过优化化期间蛋转角来提高幼的化能力和质量
Fang Chen1, Biwen Zhu2, Binbin Guo1
1Key Laboratory of Protected Agriculture Engineering in the Middle and Lower Researches of Yangtze River, Ministry of Agriculture; Laboratory of Animal Improvement and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Poultry science
|August 6, 2024
概括
优化蛋化和更大的转向角度 (75°) 改善了胚胎的发育和化能力. 一种动态的方法,在化期间改变角度,进一步提高了子的生长和生存率.
科学领域:
- 动物科学 动物科学
- 发育生物学 发展生物学
- 化技术的化技术
背景情况:
- 卵转化对于胚胎发育和成功化至关重要.
- 目前的化技术可能无法完全优化胚胎发育和化能力.
研究的目的:
- 开发一种改进的蛋化技术,使用修改的蛋转角.
- 研究扩大和变化的转角对胚胎发育,化能力和化后性能的影响.
主要方法:
- 蛋的化角度扩大 (75°对45°).
- 采用了动态转策略,在化日15日至25日之间,从75°到60°的角度变化.
- 评估了胚胎死亡率,阳膜 (CAM) 发育,化能力,幼体重和基因表达.
主要成果:
- 将转角度增加到75°加速了CAM发育,并显著降低了晚期胚胎死亡率.
- 这导致了化能力的提高,更高的健康幼率,并增加了幼的活重.
- 改变角度的策略 (75°,然后60°) 进一步降低了死亡率,最大限度地提高了化能力,增强了生长性能,并提高了化的小的体内轴基因表达.
结论:
- 一种动态的蛋转换技术,在前15天使用75°,之后使用60°,显著提高了子胚胎的生存能力和化能力.
- 这种方法增强了CAM发育,并提高了关键的与生长相关的基因 (GHRH,GHR,IGF-1) 的调节.
- 优化的转策略最大限度地提高了幼的化能力和随后的生长性能.
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