蛋白质氨酸甲基转移酶6调解了植物中的抗病毒免疫力
Qiangqiang Zhu1, Ayaz Ahmad2, Chunmei Shi1
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Cell host & microbe
|August 6, 2024
概括
植物免疫涉及蛋白质氨酸甲基转移酶6 (PRMT6) 向病毒抑制器RNA沉默 (VSR) 蛋白质. PRMT6甲基化抑制VSR,减少病毒感染并增强植物对病毒的抵抗力.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 病毒学 病毒学
- 植物免疫力 植物免疫力
背景情况:
- 病毒抑制器RNA沉默 (VSR) 蛋白质通过干扰宿主RNA沉默防御,对病毒感染至关重要.
- 植物免疫反应,包括基于NLR和自介导的途径,已经演变为准VSRs.
- 植物识别和抵消VSRs的特定分子机制尚未完全阐明.
研究的目的:
- 研究一种新的蛋白质氨酸甲基转移酶6 (PRMT6) 介导的防护机制,以对抗番茄植物中的VSR.
- 阐明PRMT6在植物免疫力中对西红布希特技病毒 (TBSV) 感染的作用.
- 确定PRMT6-VSR相互作用的分子基础及其对VSR功能的影响.
主要方法:
- 生成了PRMT6淘汰和过度表达的番茄线来评估疾病易感性.
- 进行了生物化学测定,以确认PRMT6与TBSV P19 VSR的相互作用.
- 利用位点定向突变发生来识别PRMT6甲基化向的P19中的关键氨酸残留物.
- 分析了天然番茄种群,以将PRMT6表达基因与病毒抵抗水平相关联.
主要成果:
- PRMT6淘汰番茄植物对TBSV的易感性增加,而过度表达线表现出增加的耐药性.
- PRMT6直接与TBSV P19的VSR功能相互作用并通过氨酸甲基化在R43和R115.5残留物中抑制其功能.
- 通过PRMT6介导的甲基化减少了P19二分化和小RNA结合活动,这对其VSR功能至关重要.
- 番茄种群中PRMT6表达水平的自然变化与对TBSV的差异性耐药性有显著联系.
结论:
- 通过向病毒VSRs,PRMT6充当植物免疫的关键组成部分.
- PRMT6对VSR的氨酸甲基化是一种用于植物抗病毒防御的新机制.
- 这项研究揭示了一种关键的分子相互作用,这支了植物对病毒病原体的抵抗力.
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