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相关概念视频

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The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
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Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
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滑凝对阴道微生物群采样的影响

Daphna Amitai Komem1, Rotem Hadar2, Joseph Nathaniel Paulson3

  • 1Department of Obstetrics and Gynecology, Sheba Medical Center, Affiliated With the Tel-Aviv University, Tel Hashomer, Israel. kadaphna@gmail.com.

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概括

在妇科检查中使用的滑凝可以影响阴道样品的质量,但不会改变阴道微生物组合. 为了获得准确的结果,在使用凝之前或24小时后收集样本.

关键词:
16S rRNA 测序的测序方法这是一种细菌性阴道炎.多种组合的多种组合.过早分娩 过早分娩是什么

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科学领域:

  • 微生物学 微生物学
  • 妇科 妇科医生 妇科
  • 分子生物学分子生物学

背景情况:

  • 凝滑在妇科检查中很常见,以减少不适.
  • 它在采样期间对阴道微生物组成的影响尚不清楚.

研究的目的:

  • 为了研究滑凝对阴道样品微生物组成的影响.
  • 评估带有和没有凝收集的样本的质量和微生物完整性.

主要方法:

  • 收集了来自31名怀孕妇女的143个阴道样本.
  • 采集了带有和没有凝的样本,立即冷,或在室温下5小时后,以及24小时后的样本.
  • 微生物分析使用16S安普利康测序进行.

主要成果:

  • 凝滑对样品质量产生了负面影响,其中一半的凝样品没有达到加工限制.
  • 这种对质量的影响在24小时后会减弱.
  • 当样品遇到测序后的过器时,凝不会改变微生物的组成,种群的丰富性或多样性.

结论:

  • 阴道样本采集方法,特别是凝的使用,可能会影响样本质量.
  • 凝滑似乎不会改变阴道微生物组的组成本身.
  • 建议在凝涂抹前或涂抹后24小时收集样本,以确保数据的完整性.