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相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Pre-mRNA Processing: RNA Splicing01:36

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Regulation of Expression at Multiple Steps01:23

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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pre-mRNA Processing02:01

pre-mRNA Processing

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
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在大麦 (Hordeum vulgare) 的发芽过程中,盖结合复合物调节了ABA-响应的转录拼接.

Ewa Sybilska1, Anna Collin1, Bahareh Sadat Haddadi2

  • 1Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.

Scientific reports
|August 6, 2024
PubMed
概括

结复合体 (CBC) 通过与酸 (ABA) 相互作用来调节种子发芽. 双重突变体表现出ABA不敏感性,与单一突变体不同,显示出CBC.

关键词:
阿巴阿巴阿巴是什么意思另一个替代拼接.大麦大麦,大麦大麦,大麦大麦,大麦大麦.顶部绑定复杂的复合体胚胎 胚胎是一个胚胎.发芽 发芽 发芽 发芽转录组 转录组就是一个转录组.

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科学领域:

  • 植物生物学 植物生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 种子发芽是一个重要的发育过程.
  • 酸 (ABA) 是一个关键的植物激素,调节种子休眠和发芽.
  • 由CBP20和CBP80组成的盖结合复合体 (CBC) 参与RNA处理.

研究的目的:

  • 调查CBC在调节酸 (ABA) 对大麦种子发芽的影响中的作用.
  • 阐明CBC在ABA信号传递中的功能背后的分子机制.

主要方法:

  • 在编码CBC成分 (CBP20和CBP80) 的基因中利用单个和双重大麦突变.
  • 在突变系上进行了转录组和代谢组分析.
  • 在不同的ABA度下评估了发芽表型.

主要成果:

  • 双重突变的hvcbp20.ab/hvcbp80.b在发芽期间表现出ABA不敏感性.
  • 单个突变者表现出ABA过敏,与双重突变的表型形成鲜明对比.
  • 转录组和代谢组分析揭示了基因表达,剪接和CBC,ABA和布拉西诺体 (BR) 信号之间识别的交叉声调的显著变化.

结论:

  • 在大麦种子发芽期间,CBC在调节ABA敏感性方面发挥着关键而复杂的作用.
  • CBC的功能对于适当的ABA信号传输至关重要,CBP20和CBP80的角色是不同的.
  • 该研究强调了一个复杂的监管网络,涉及CBC,ABA和BR通路,影响植物发育.