来自共生微生物的外膜囊泡有助于海绵Tedania sp. 通过调节诱导亡因子 (AIF) 的表达水平来调节发育过程
Kai Wang1, Chenzheng Jia2, Beibei Zhang3
1College of Ocean and Earth Science of Xiamen University, Xiamen, 361005, China. 22320200155959@stu.xmu.edu.cn.
Communications biology
|August 6, 2024
概括
细菌外膜囊泡 (OMVs) 通过改变亡诱导因子 (AIF) 基因表达来影响海洋海绵的沉积. 这项研究表明,OMV可以携带AIF小RNA,直接影响海绵的发育.
科学领域:
- 海洋生物学 海洋生物学
- 发育生物学是发展生物学.
- 微生物学 微生物学
背景情况:
- 幼虫定居是海洋海绵发育的一个关键阶段.
- 来自Tenacibaculum mesophilum的细菌外膜囊泡 (OMV) 影响海绵沉积物,其影响取决于度.
- 以前的研究表明,细菌性OMV和海绵幼虫定居率之间存在联系.
研究的目的:
- 调查细菌OMV调节海洋海绵幼虫定居点的分子机制.
- 在定居阶段识别受OMV暴露影响的宿主基因.
- 探索OMV中小RNAs在媒介宿主微生物相互作用中的作用.
主要方法:
- 对暴露于过海水 (FSW) 的海绵幼虫的比较转录组分析与用OMV补充的FSW (FSW-OMV) 相比.
- 定量实时PCR验证细胞亡诱导因子 (AIF) 基因表达的变化.
- RNA干扰 (RNAi) 来评估AIF在幼虫定居中的功能作用.
- 细菌OMVs的小RNA (sRNA) 测序.
主要成果:
- 细菌OMV显著调节了海绵幼虫中诱导亡因子 (AIF) 的表达.
- 与对照组相比,AIF基因表达在靠近定居点 (SE) 的幼虫中减少.
- 通过RNAi介导的AIF降低了幼虫结算率,证实了它的抑制作用.
- 在OMV中发现了丰富的30核酸AIF小RNA (sRNA).
结论:
- 细菌OMV通过调节宿主AIF的表达,影响海洋海绵的结算.
- 这些发现表明一种新的机制,即细菌衍生的OMVs提供功能性sRNA来影响宿主发育.
- 这突出了细菌OMV及其载荷在关键生命过渡期间调解海绵-微生物相互作用的直接作用.
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