结核病皮肤测试阳性和皮肤测试阴性牛之间的广泛差异性DNA甲基化
Sajad A Bhat1, Alia Parveen1, Eamonn Gormley2
1UCD School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, D04 V1W8, Ireland.
BMC genomics
|August 6, 2024
概括
牛DNA甲基化的表观遗传变化与牛结核病 (bTB) 试验结果有关. 不同的甲基化基因,特别是在免疫路径中,可能会影响诊断结果和宿主对Mycobacterium bovis感染的反应.
科学领域:
- 兽医免疫学 兽医免疫学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 动物健康 动物健康
背景情况:
- 由Mycobacterium bovis引起的牛结核病 (bTB) 对农业和人类健康构成重大风险.
- 目前的bTB诊断依赖于通过结核素皮肤试验检测到的细胞介导免疫反应.
- 表观遗传修饰,特别是DNA甲基化,可以影响牛的免疫反应和诊断测试结果.
研究的目的:
- 调查不同牛结核病 (bTB) 诊断状态的牛的DNA甲基化概况.
- 为了识别与bTB测试结果相关的差异甲基化区域 (DMR) 和基因.
- 探索表观遗传修饰对bTB易感性和诊断准确性的潜在作用.
主要方法:
- 全基因组双硫酸序列测定 (WGBS) 用于分析bTB测试阳性和测试阴性牛的外周血液中的DNA甲基化.
- 统计分析确定了差异甲基化区域 (DMR) 和差异促进甲基化基因 (DPMG).
- 进行了KEGG通路分析,以了解已识别的DMRs的功能影响.
主要成果:
- 全球甲基化概况相似,但在两组之间确定了223个DMR和159个DPMG,在SICTT阳性牛中超甲基化的流行率较高.
- 在DMR中的关键基因包括IL1R1和MHC相关基因 (BOLA,BOLA-DQB).
- 丰富的途径涉及和MAPK信号传递,以及新陈代谢. 在IFN-γ阳性牛群中,DMRs影响IL10RA,IL17F和宿主防御.
结论:
- 不同的DNA甲基化与牛的bTB诊断测试结果有关.
- 特定的免疫基因位点显示了与bTB状态相关的改变甲基化模式.
- DNA甲基化程度可能会影响宿主免疫反应和bTB诊断的有效性.
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