外体circ-0100519通过通过USP7/NRF2轴诱导M2巨细胞极化促进乳腺癌的进展
Minyu Zhuang1, Xiaoqiang Zhang2, Jie Ji1
1Breast Disease Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
Clinical and translational medicine
|August 7, 2024
概括
外体circ-0100519通过诱导M2类巨细胞促进乳腺癌 (BC) 转移. 这种循环RNA (circRNA) 是BC诊断和预后的潜在生物标志物,HIF-1α抑制剂提供治疗途径.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 乳腺癌 (BC) 是全球女性的主要健康问题.
- 循环RNAs (circRNAs) 与BC进展和瘤微环境 (TME) 调制有关.
研究的目的:
- 研究circ-0100519在BC发育中的作用.
- 探索外体环RNAs作为BC.的诊断和预后生物标志物的潜力.
- 为了确定BC治疗的治疗点.
主要方法:
- 高通量测序和生物信息学用于circRNA分析.
- 使用电子显微镜对外体的表征.
- 对于circ-0100519的体外和体内功能测定.
- 通过Western blotting,RNA pull-down和 luciferase测试来研究分子机制.
主要成果:
- 在BC组织中,Circ-0100519被上调,并与预后不佳有关.
- 外体环-0100519通过诱导M2巨细胞极化促进BC细胞入侵和转移.
- Circ-0100519充当了巨细胞中USP7-NRF2相互作用的支架,增强了NRF2的二氧化化.
- 在HIF-1α上升调节circ-0100519的转录.
结论:
- 外基因组circ-0100519作为BC诊断和预后的一个有前途的生物标志物.
- HIF-1α抑制剂PX-478为BC提供了一个潜在的治疗策略.
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