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在实验性急性肺炎中,ARDS微环境通过VEGF增强MSC诱导的修复

Courteney Tunstead¹, Evelina Volkova¹, Hazel Dunbar¹

¹Cellular Immunology Lab, Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. Kildare, Ireland.

Molecular therapy : the journal of the American Society of Gene Therapy

|August 7, 2024

概括

在高炎症环境中预先授权介质细胞 (MSC) 增强它们对急性肺炎的疗效. 这种方法改善了小鼠的肺屏障完整性和临床结果,建议针对ARDS亚型量身定制的MSC治疗.

科学领域:

再生医学是一种再生医学.
免疫学免疫学免疫学
细胞疗法细胞疗法

背景情况:

介酶体 stromal 细胞 (MSCs) 在治疗急性呼吸困扰综合征 (ARDS) 等炎症性疾病方面表现出有限的疗效.
ARDS呈现出明显的低-和超-炎症表型,可能会影响治疗反应.
细胞因子介导激活可以增强MSC的治疗潜力.

研究的目的:

调查是否在超炎症性ARDS环境中预许可MSC可以提高其在急性肺炎 (ALI) 中的治疗效果.
为了确定不同的ARDS炎症状态是否会对MSC功能产生不同的影响.

主要方法:

来自低和高炎症ARDS患者的血清 (基于IL-6水平) 用于许可MSCs.
用许可血清或健康的对照血清培养MSC.
在实验室和体内评估了MSC-CM对肺上皮细胞和ALI小鼠模型的影响.

主要成果:

超炎症性ARDS授权的MSC-CM (MSC-CM_Hyper) 通过血管内皮生长因子 (VEGF) 增强了紧结表达和肺上皮屏障完整性.
低和高炎症性ARDS授权的MSC-CM降低了ALI小鼠支气管支气管洗液中的IL-6和TNF-α水平.
只有MSC-CM_Hyper显著降低了ALI小鼠的肺透性,体重减轻和改善临床评分.

结论:

ARDS的炎症环境通过差异调节MSC的细胞保护和免疫调节功能.
在高炎症环境中预先许可MSC可以提高其对ALI的治疗潜力.
根据ARDS表型量身定制MSC治疗可能会改善治疗结果.

关键词:

在 ARDS 中,ARDS 是指 ARDS 的类型.在MSC中,MSC是MSC.这就是SARS-CoV-2病毒.在VEGF中,VEGF是VEGF.透性透性的

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