雌性在交配期间转移的雄性mRNA进行翻译
Luciano M Matzkin1,2,3, Jeremy M Bono4, Helen K Pigage4
1Department of Entomology, University of Arizona, Tucson, AZ, USA.
iScience
|August 7, 2024
概括
男性的精液RNA被雌性果转化,产生影响生殖和性冲突的蛋白质. 这揭示了许多物种在交配后相互作用的新型分子机制.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 许多物种的精液中含有RNA,但其在雌性体内的功能在很大程度上是未知的.
- 了解两性之间的分子相互作用对繁殖成功和进化至关重要.
研究的目的:
- 调查男性精液RNA是否具有功能性,并由 *Drosophila* 的雌性进行翻译.
- 为了识别和描述由雌性从精液RNA翻译的男性衍生蛋白质.
主要方法:
- 开发一种新型蛋白质组学方法,变种启用SILAC蛋白质组学分析 (VESPA).
- 应用VESPA来量化女性生殖系统中男性衍生的蛋白质.
- 使用淘汰实验来评估已识别的蛋白质的功能作用.
主要成果:
- 在女性下生殖道中鉴定了67种男性衍生,女性转化蛋白 (mdFTPs).
- 许多mdFTP具有与生殖过程相关的预测功能.
- 发现mdFTPs影响了受精成功和受精反应质量的形成/持久性.
结论:
- 男性精液RNA可以被女性翻译,从而产生影响生殖的功能性蛋白质.
- 这些发现揭示了先前未被识别的后交配分子相互作用机制,影响性冲突和合作.
- 这一发现对理解各种物种中性别之间的生殖策略和分子通信有着广泛的影响.
相关概念视频
Regulated mRNA Transport
6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
Initiation of Translation
32.1K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
32.1K
Regulation of Expression Occurs at Multiple Steps
22.6K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.6K
Dosage Compensation
6.1K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.1K
Regulation of Expression at Multiple Steps
879
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
879
pre-mRNA Processing
52.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
52.8K


