在真核生物中,类固醇5α-减少酶家族的进化,分类,结构和功能多样化
Khawar Ali1, Wenjuan Li1, Guang Wu1
1College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, China.
Heliyon
|August 7, 2024
概括
这项研究追踪了类固醇5α-减少酶 (SRD5A) 基因的进化历史,揭示了它们在原生体中的古代起源和在真核生物中的多样化. 它确定了一个新的植物子类,类似DET2,具有未知的功能.
科学领域:
- 进化生物学 进化生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 类固醇5α-减少酶 (SRD5A) 蛋白质,包括哺乳动物SRD5A1/2和植物DET2,对于类固醇激素生物合成至关重要.
- 这些酶具有相同的功能同质性和共同的进化起源,在各自的王国中催化出不同的类固醇减少.
研究的目的:
- 调查 SRD5A 基因家族在真核生物中的进化轨迹,结构和功能同质性.
- 确定重复的SRD5A基因的起源并了解其功能多样化.
主要方法:
- 来自不同真核生物种的SRD5A家族基因的系遗传学分析.
- 对SRD5A1/2,DET2和新发现的DET2-like (DET2L) 基因进行比较分析.
主要成果:
- 原生体代表SRD5A基因家族的祖先起源,尽管一些血统显示基因丢失.
- 基因重复事件是基因特异性的,导致SRD5A基因拷贝数的变化 (例如,在*C. elegans*中多个基因,在*D. melanogaster*中没有任何基因).
- 一个新的植物亚类,DET2-like (DET2L) 被确定,但它的功能仍然不清楚,因为它没有拯救Arabidopsis det2*突变.
结论:
- SRD5A基因家族有古老的起源,并在真核生物进化过程中经历了显著的多样化和重复.
- 对于SRD5A同类的功能作用,特别是植物中新发现的DET2L亚类,需要进一步研究.
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