优化由植物生长促进真菌产生的酸,在响应表面方法的帮助下
Palak Arora1,2, Rubeena Tabssum1,2,3, Ajai P Gupta4,2,3
1Plant Sciences and Agrotechnology Division, India.
Heliyon
|August 7, 2024
概括
内生菌Diaporthe terebinthifolli GG3F6增强了植物的生长和糖素的生产. 使用响应表面方法 (RSM) 优化醇酸 (IAA) 生产可促进植物发育和有益化合物积累.
科学领域:
- 微生物学 微生物学
- 植物科学 植物科学
- 生物技术是生物技术.
背景情况:
- 类植物,如真菌,在植物微生物相互作用中起着至关重要的作用.
- 由内细胞产生的酸 (IAA) 显著影响植物的生长和发育.
- 甘 (Glycyrrhiza glabra) 是一种有价值的药用植物,含有各种生物活性化合物.
研究的目的:
- 用响应表面方法 (RSM) 来优化内真菌Diaporthe terebinthifolli GG3F6的印醇酸 (IAA) 生产.
- 为了研究IAA产生的内菌GGG3F6对Glycyrrhiza glabra.生长和生物化学组成的影响.
- 探索D. terebinthifolli作为生物刺激剂的潜力,以增强糖素积累.
主要方法:
- 从Glycyrrhiza glabra根茎中分离出内菌GG3F6的内菌.
- 使用响应表面方法 (RSM) 优化IAA的产量,使用不同的酸盐,pH和化时间.
- 在GG3F6和GG3F6的实验室内G. glabra植物的共同培养以及植物生长参数和生物化学含量的分析.
主要成果:
- 在优化条件下,IAA积累增加了1.1倍,达到121.20μg/mL.
- RSM模型显示了独立变量对IAA生产的显著影响 (p < 0.0001).
- GG3F6治疗在体外增强了G. glabra的根和芽生长,总和黄酸含量,特别是糖含量增加了2.1倍.
结论:
- 响应表面方法有效优化了IAA产量通过Diaportthe terebinthifolli GG3F6.6.
- 体GG3F6作为一种强大的生物刺激剂,促进植物生长,增强Glycyrrhiza glabra.中的关键代谢物积累.
- 这项研究表明了D. terebinthifolli在农业和制药应用中的潜力,特别是提高糖素的生产.
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