相关实验视频

Updated: Jun 17, 2025

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Published on: August 8, 2019

高通量前骨质细胞球体通过甲状腺激素信号通路提升纤维细胞生长因子23

Jie Jiang¹, Jingxian Zhu¹, Haojie Lin¹

¹State Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Tissue engineering. Part C, Methods

|August 7, 2024

概括

从前骨质细胞中开发出3D球体显著增加了纤维细胞生长因子23 (FGF23) 的产生. 这种在球状体中增强的FGF23表达有助于研究骨质平衡调节和确定新的治疗点.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

纤维细胞生长因子23 (FGF23) 对于酸盐和维生素D的新陈代谢至关重要,影响骨平衡.
目前的二维细胞培养表明FGF23的生产有限,阻碍了对其调节机制和治疗应用的研究.

研究的目的:

开发一个高通量3D体外系统,以提高FGF23的生产.
调查前骨质细胞球状体中FGF23调节的基础机制.

主要方法:

鼠骨前细胞MC3T3-E1细胞在非粘附的微中培养成球形.
在基因和蛋白质水平上分析了FGF23的产量.
RNA测序和西部涂抹确定了副甲状腺激素 (PTH) /PTH1R信号通路的作用.
使用小分子和短毛RNA (shRNA) 抑制了PTH信号传递.

主要成果:

与2D培养相比,MC3T3-E1球体表现出明显更高的FGF23基因和蛋白质表达.
增强的FGF23产生与PTH/PTH1R信号通路的激活有关.
抑制PTH信号传递有效地降低了球状体中的FGF23产量.

结论:

高通量球形形成是一种有效的方法来刺激前骨质细胞中的FGF23表达.
3D球形系统为研究FGF23调节提供了一个有价值的平台.
这种3D模型在与FGF23相关的研究中具有药物发现和目标识别的潜力.

关键词:

三维文化是3D文化.联邦政府财政基金23 PTHTH PTH 这是一个很好的方法.细胞球形体是细胞球形体.骨质母细胞 (osteoblasts) 是一个骨质细胞.

更多相关视频

Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Published on: May 19, 2023

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Hormones and Bone Tissue

Hormones and Bone Tissue

The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...

The Parathyroid Glands

The Parathyroid Glands

The two pairs of parathyroid glands embedded within the posterior surface of the thyroid gland are restricted by a dense capsule around them. These glands comprise two distinct cell populations—parathyroid oxyphil and parathyroid principal cells- pivotal in calcium homeostasis.
Oxyphil cells, whose functions remain elusive, emerge during late puberty, adding a layer of complexity to the parathyroid gland's intricacies. In contrast, principal parathyroid cells undertake a vital role by...