N6-甲基氨酸RNA修饰调节了棉花中的光周期灵敏度
Ying He1, Zhanfeng Si1, Gaofu Mei2
1Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
Plant physiology
|August 7, 2024
概括
N6-甲基氨酸 (m6A) 的修改调节了棉花的开花时间. GhALKBH5脱甲基酶通过改变开花基因的m6A水平来影响光周期敏感性,从而影响了花过渡.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- N6-甲基氨酸 (m6A) 是一个关键的表观遗传修饰调节基因表达.
- m6A的修改涉及到各种生物过程,包括植物的花过渡.
- 在棉花光周期敏感性中m6A的作用尚不清楚.
研究的目的:
- 调查m6A修饰在棉花光周期敏感性中的作用.
- 为了确定关键的基因和调节机制,参与m6A介导的光周期花期在Gossypium hirsutum.
主要方法:
- 对光周期敏感和不敏感的棉花品种进行了全转录组的m6A测序.
- 进行了GHALKBH5和关键光周期性开花基因的基因沉默.
- 逆转录定量PCR (RT-qPCR) 用于分析基因表达水平.
主要成果:
- 在棉花品种之间观察到m6A甲基化模式的显著差异,与光周期灵敏度相关.
- 脱甲基酶GhALKBH5被确定为m6A水平的关键调节者.
- 沉默GhALKBH5通过降低光周期性开花基因 (GhADO3,GhaGL24,GhFT1) 的调节延迟了开花.
结论:
- 光周期性开花转录的GhALKBH5-介导的m6A脱甲基化是调节棉花光周期敏感性的关键机制.
- 这项研究揭示了一种新的表观遗传调节途径,控制了Gossypium hirsutum的开花时间.
相关概念视频
RNA Editing
9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
RNA Stability
33.4K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.4K
Transcription Attenuation in Prokaryotes
15.2K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.2K
Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
Regulation of Expression at Multiple Steps
879
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
879
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K


