同时的p53和p16免疫化用于分子分类的头部和部状细胞癌
Pihla Pakkanen1, Antti Silvoniemi2, Katri Aro1
1Department of Otorhinolaryngology - Head and Neck Surgery, University of Helsinki, Helsinki University Hospital, Helsinki, Finland.
Head and neck pathology
|August 7, 2024
概括
同时的p16和p53免疫组织化学染色准确地将大多数头部和部状细胞癌 (HNSCC) 分类为与HPV相关的或与HPV独立的,从而可能减少进一步分子测试的需要.
科学领域:
- 在瘤学瘤学.
- 病理学 病理学 病理学
- 分子生物学分子生物学
背景情况:
- 头部和部状细胞癌 (HNSCC) 需要准确的分类.
- 人类乳头瘤病毒 (HPV) 状态是HNSCC预后和治疗的关键因素.
- 区分HPV相关的 (HPV-A) 和HPV独立的 (HPV-I) HNSCC是必不可少的.
研究的目的:
- 评估p16和p53同时进行免疫组织化学染色 (IHC) 的有效性,以对HNSCC进行分类.
- 为了将p53 IHC模式与TP53突变状态,p16 IHC阳性和HPV状态相关联.
- 确定这种IHC方法是否可以可靠地区分HPV-A和HPV-I瘤.
主要方法:
- 31例HNSCC病例被染色为p16和p53使用IHC.
- 对所有病例进行了下一代测序 (FoundationOne©CDx).
- 在23个案例中进行了HPV in-situ杂交 (ISH).
- p53 IHC模式被分为野生型 (wt) 或异常型 (abn).
主要成果:
- 同时的p16和p53IHC在31例中28例提供了直接分类.
- 10个病例被归类为HPV-A (p16+/p53wt) 和18个被归类为HPV-I (p16-/p53abn).
- 分子测试解决了模两可的病例,证实了HPV状态和TP53突变相关性.
结论:
- 同时的p16和p53 IHC是分类HNSCC的可靠方法.
- HPV-A HNSCC通常显示p16阳性和p53野生型染色 (基底节约或类似于零).
- HPV-I HNSCC通常表现出p16负面性和p53异常 (突变模式) 表达.
- 这种IHC方法可以限制需要额外的分子HPV或突变测试.
更多相关视频
07:43Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
Published on: July 29, 2017
9.6K
06:57Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
Published on: June 14, 2019
10.4K
相关概念视频
Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
