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相关概念视频

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

16.2K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
16.2K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

20.9K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
20.9K
Transcription Initiation01:47

Transcription Initiation

16.3K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.3K
Reporter Genes02:11

Reporter Genes

11.3K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.3K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

29.4K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.4K
Bacterial Transcription01:53

Bacterial Transcription

28.1K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
28.1K

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相关实验视频

Updated: Jun 17, 2025

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

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迅速预测多个 prokaryotes 中的促使者.

Qimeng Du1, Yixue Guo2, Junpeng Zhang1

  • 1School of Engineering, Air-Space-Ground Integrated Intelligence and Big Data Application Engineering Research Center of Yunnan Provincial Department of Education, Dali University, Dali, 671003, China.

Interdisciplinary sciences, computational life sciences
|August 7, 2024
PubMed
概括

快速准确地预测了16个物种中的 prokaryotic 促销者,改善了基因功能研究. 这种方法通过识别促进子序列动机来提高预测的解释性.

关键词:
这就是K-MER.相反的学习学习.多层感知多层感知.Prokaryotic 促销者 促销者 促销者 促销者 促销者

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
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Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

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相关实验视频

Last Updated: Jun 17, 2025

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
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Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

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科学领域:

  • 基因组学和生物信息学
  • 分子生物学分子生物学

背景情况:

  • 准确预测 prokaryotic 促销者对于理解基因调节和功能至关重要.
  • 现有的方法往往缺乏跨物种适用性和解释性,因为它们对序列动机的关注有限.

研究的目的:

  • 开发一种通用方法,即Prompt,用于预测多种 prokaryotic 物种中的促进体.
  • 通过结合序列动机识别来提高推动者预测的解释性.

主要方法:

  • 快速集成回归基于选择的k-mer (RSK),对比学习 (CL) 和多层感知 (MLP).
  • 一个投票策略将预测分为高信心和低信心集.
  • 该方法在16种 prokaryotic 种类的促进者预测任务上进行了评估.

主要成果:

  • 在16种 prokaryotes 的高可靠性数据集上,Prompt 实现了超过 80% 的准确性 (准确性,马修斯相关系数).
  • 在16种 prokaryotes 中,12种的精度超过了 90%.
  • 与现有的预测方法相比,Prompt表现出更高的性能.

结论:

  • 提示提供了一种广义和可解释的方法来预测 prokaryotic 促销者.
  • 该方法的高精度和识别动机的能力将有助于阐明基因功能和调节机制.
  • 提示是公开可用的,并准备推进 prokaryotic 研究.