相关实验视频

Updated: Jun 17, 2025

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

一个CRISPR/RfxCas13d介导的战略,用于有效的RNA淘汰小鼠胚胎发育

Lin Zhang¹, Shi-Meng Cao², Hao Wu²

¹State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China.

Science China. Life sciences

|August 7, 2024

概括

这项研究引入了一种结合CRISPR-RfxCas13d和半克隆的新方法,以抑制特定的RNA水平 in vivo. 这种技术有效地揭示了Sfmbt2 mRNA,Fendrr lncRNA和circMan1a2在胚胎发育中的关键作用.

科学领域:

分子生物学分子生物学
发育生物学发展生物学
遗传学遗传学是一个

背景情况:

不同的RNA类 (mRNA,lncRNA,circRNA) 对于发育和疾病至关重要.
由于缺乏有效的RNA操纵工具,对RNA in vivo功能的理解有限.

研究的目的:

开发和验证一种用于活生物体中向RNA抑制的新系统.
研究特定RNA (Sfmbt2 mRNA,Fendrr lncRNA,circMan1a2) 在胚胎发育中的作用.

主要方法:

使用CRISPR-RfxCas13d系统进行RNA向.
采用类似精子干细胞介导的半克隆,用于基因传递和表达抑制.
与Sfmbt2 mRNA,Fendrr lncRNA和circMan1a2.2的表达受到干扰.

主要成果:

成功抑制了目标mRNA,lncRNA和circRNA分子的表达.
证明了这些RNA在胚胎发育中的关键作用,由死亡率和发育迟缓等表型证明.
在抑制特定的RNA点时观察到胚胎再吸收.

结论:

开发的CRISPR-RfxCas13d和半克隆方法提供了一个强大的工具,用于在体内制向RNA.
这种方法使得在没有遗传修饰的情况下对特定RNA的功能性研究成为可能.
这项研究强调了Sfmbt2 mRNA,Fendrr lncRNA和circMan1a2在成功的胚胎发育中的重要作用.

关键词:

在CRISPR-RfxCas13d中使用CRISPR.通过RNA的淘汰,RNA的淘汰.胚胎发育过程中的胚胎.一个半克隆技术.类似精子干细胞的精子干细胞.

更多相关视频

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

相关概念视频

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...