相关实验视频

Updated: Jun 17, 2025

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

3.0K

一个CRISPR/RfxCas13d介导的战略,用于有效的RNA淘汰小鼠胚胎发育

Lin Zhang1, Shi-Meng Cao2, Hao Wu2

  • 1State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China.

Science China. Life sciences
|August 7, 2024
PubMed
概括

这项研究引入了一种结合CRISPR-RfxCas13d和半克隆的新方法,以抑制特定的RNA水平 in vivo. 这种技术有效地揭示了Sfmbt2 mRNA,Fendrr lncRNA和circMan1a2在胚胎发育中的关键作用.

关键词:
在CRISPR-RfxCas13d中使用CRISPR.通过RNA的淘汰,RNA的淘汰.胚胎发育过程中的胚胎.一个半克隆技术.类似精子干细胞的精子干细胞.

更多相关视频

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

7.7K
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

9.7K

相关概念视频

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K