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牙签DNA提取与手持LAMP微流体平台相结合,用于简单快速的肉类认证

Bin Xiao¹, Tianping Zhou², Nan Wang¹

¹Institute of Quality Standard & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

|August 7, 2024

概括

一种新方法使用牙签,微流体芯片和智能手机来快速且负担得起地检测肉类改. 这个便携式系统可以在40分钟内以每次测试不到一美元的价格识别伪造者.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
生物技术是生物技术.

背景情况:

肉类改是一个重大的全球性问题.
现有的检测方法往往是缓慢的,昂贵的,或需要实验室设置.
需要快速,现场和具有成本效益的测试解决方案.

研究的目的:

开发一种快速,廉价,便携式的系统,用于检测多种肉类改.
为了创建一个一分钟的牙签基DNA提取方法.
将其与手持式微流体芯片和智能手机控制的便携式分析仪相集成.

主要方法:

牙被用于从肉样品中释放和吸附DNA.
一个手持的微流体芯片促进了DNA化和流体处理.
一个由智能手机应用程序控制的便携式分析仪提供了加热,信号检测和结果读取.

主要成果:

开发的系统成功检测到模拟的造肉样品.
检测极限达到低至1%的伪造 (英国标准).
整个过程,从样品到结果,在40分钟内完成.

结论:

拟议的系统提供了一种简单,用户友好和快速的方法,用于现场检测肉类改.
低成本 (每次测试不到1美元) 和便携性使其适合广泛应用.
这项技术解决了全球对可访问的肉类真实性测试的需求.

关键词:

分析仪分析仪应用程序应用程序应用程序手持式微流体芯片 - - 微流体芯片一个灯的灯光.肉类的改肉类的改在护理场所进行检测.一个牙棒.

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