相关实验视频
Updated: Jul 17, 2026

10:53
Microgavage of Zebrafish Larvae
Published on: February 20, 2013
16.9K
在斑马鱼幼虫中,阿维尔麦克丁诱导了血管损伤:与线粒体介导的亡和VEGF/Notch信号通路有关
Wei-Guo Wang1, Xu-Feng Jiang1, Cheng Zhang2
1Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Journal of hazardous materials
|August 7, 2024
概括
杀虫剂阿维雷克会在斑马鱼幼虫中引起血管损伤,影响血管大小和细胞数量. 这种血管毒性可能涉及线粒体功能障碍和VEGF/Notch信号通路.
科学领域:
- 毒理学 毒理学 毒理学
- 发展生物学 发展生物学
- 生态毒理学 生态毒理学
背景情况:
- 自20世纪90年代以来,Avermectin是一种广泛使用的杀虫剂,已引起人们对其对非标生物的安全性表示担忧.
- 血管健康对器官功能至关重要,但对阿弗梅克对血管系统的影响的研究有限.
- 斑马鱼幼虫提供了适合血管研究的模型,因为它们的透明身体和良好的 vasculature.
研究的目的:
- 为了研究在斑马鱼幼虫中阿弗梅克丁的潜在血管毒性.
- 为了识别由阿维尔麦克丁暴露引起的血管损伤的特定形式.
- 阐明阿弗梅克诱导的血管损伤背后的分子机制.
主要方法:
- 斑马鱼幼虫暴露于阿弗米克丁.
- 整个身体的血管系统的形态评估.
- 细胞内皮细胞数量和亡的量化.
- 分析与VEGF/Notch信号相关的线粒体功能和基因表达.
主要成果:
- 在斑马鱼幼虫中,阿维尔麦丁诱导了多种组织的显著血管损伤,包括直径,面积和丰度的减少.
- 暴露导致内皮细胞数量的减少和血管系统内的亡的增加.
- 观察到线粒体功能受损和线粒体介导的亡.
- 在VEGF/Notch信号通路中发现了异常基因表达.
结论:
- 在斑马鱼幼虫中,阿维尔麦克丁表现出明显的血管毒性.
- 观察到的血管损伤可能与线粒体功能障碍和亡有关.
- VEGF/Notch信号通路在阿维美克丁诱导的血管毒性中起着重要作用.
- 这些发现凸显了对非标生物体中阿弗梅克安全性的进一步评估的必要性.
相关概念视频
Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Mechanism of Angiogenesis
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

