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相关概念视频

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

5.9K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
5.9K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

366
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
366
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

206
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
206
SDS-PAGE01:27

SDS-PAGE

27.7K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
27.7K

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相关实验视频

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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
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简化集成蛋白质异电聚焦,使用微流体纸质设备.

Geovana M Mendes1, Fanny d'Orlye2, Laura Trapiella-Alfonso2

  • 1Chimie ParisTech, PSL University, CNRS 8060, Institute of Chemistry for Life and Health Sciences, 75005 Paris, France; Instituto de Química, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil.

Journal of chromatography. A
|August 7, 2024
PubMed
概括
此摘要是机器生成的。

一个新的基于纸张的微型设备使用同电聚焦 (IEF) 实现了强大的蛋白质分离. 这种低成本的平台集成了3D打印组件,以实现高效和多功能蛋白质组分析.

关键词:
用3D打印的持有器进行3D打印.葡萄糖醇 (Glycerol) 是一种糖醇.隔离电的聚焦方式微流体基于纸张的微流体.蛋白质组学是指蛋白质组学.

更多相关视频

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing IEF Method
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On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
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相关实验视频

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07:58

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Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing IEF Method
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On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
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科学领域:

  • 生物材料科学 生物材料科学
  • 分析化学 分析化学
  • 微流体学 微流体学

背景情况:

  • 蛋白质分离对于蛋白质组学至关重要.
  • 现有的方法可能是复杂和昂贵的.
  • 基于纸张的微流体设备提供了低成本,便携式分析的潜力.

研究的目的:

  • 开发一个集成的,坚固的基于纸张的微设备,通过同电聚焦 (IEF) 来分离蛋白质.
  • 优化设备设计和IEF参数,以实现高效的蛋白质分离.
  • 为了证明平台对现实世界样本的适用性.

主要方法:

  • 开发一个3D打印的支架,集成一个分离通道,水库和电极.
  • 优化纸质基板,储设计和水/糖分离介质.
  • 描述pH梯度稳定性,分离效率和分辨率.
  • 该设备用于分析加的唾液样本的应用.

主要成果:

  • 在玻璃纤维纸质基板上实现了稳定的pH梯度.
  • 水/糖醇介质减少了蒸发,并支持了蛋白质的分离.
  • 在25分钟内实现了高效的蛋白质分离.
  • 该装置在应用于唾液样本时显示了最小的矩阵效应.

结论:

  • 开发的基于纸张的IEF微设备是强大的,低成本的,多功能.
  • 它提供了有效的蛋白质分离,适合蛋白质组学.
  • 该平台有可能用于样品预处理和集成到lab-on-a-chip系统中.