一种多功能和高效的方法来检测tRNA衍生碎片

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Mei Yang1, Yongzhen Mo2, Daixi Ren1

  • 1NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China; Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.

Molecular and cellular probes
|August 7, 2024
PubMed
概括

研究人员开发了一种新方法,通过多化所有tRF来检测tRNA衍生碎片 (tRF). 这允许同时进行逆转录和qPCR,为tRF表达分析节省时间和资源.

关键词:
一致的碎片是同类的碎片.复合 (A) 反向转录方法定量聚合酶连锁反应的量化反应.茎环逆转录方法的逆转录方法由tRNA衍生的片段 (tRFs)

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