优化使用Box-Behnken设计的fucoidan提取及其潜在的生物活性
Mostafa El-Sheekh1, Eman A Alwaleed2, Wafaa M A Kassem2
1Botany Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt.
International journal of biological macromolecules
|August 7, 2024
概括
这项研究优化了从棕色海藻中提取福科伊丹的提取方法,从帕迪纳·帕沃尼卡 (Padina pavonica) 获得了最高的量. 富科伊丹显示出显著的抗氧化,抗癌和抗微生物特性,特别是来自Dictyota二分类.
科学领域:
- 海洋生物技术 海洋生物技术
- 自然产品化学 自然产品化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 富科伊丹是一种来自棕色海藻的硫化多糖,具有显著的生物活性.
- 优化福科伊丹提取对于最大限度地提高其各种应用的产量和有效性至关重要.
研究的目的:
- 为了优化从Turbinaria turbinata中提取fucoidan,使用一个盒式模拟设计 (BBD).
- 为了评估从不同棕色海藻物种中提取的fucoidan的生物活动.
主要方法:
- 盒子-Behnken设计 (BBD) 用于优化pH值,温度和提取时间用于fucoidan提取.
- 福利埃变换红外光谱 (FTIR),1H核磁共振 (NMR) 和高性能液态染色学 (HPLC) 用于表征.
- 进行了测试,以评估抗氧化剂,抗瘤,抗微生物和抗凝活动.
主要成果:
- 优化的提取参数为pH3.44,82.26°C和60分钟,最大限度地提高了富可伊丹产量和硫酸盐含量.
- 帕迪纳·帕沃尼卡 (Padina pavonica) 的富科伊丹产量最高 (40.76 ± 4.04% DW).
- 来自Dictyota dichotoma和Sargassum cinereum的Fucoidan表现出最高的抗氧化,抗癌和抗凝活动,与较高的硫酸盐含量相关.
结论:
- 优化提取显著提高了富可伊丹产量和硫酸盐含量.
- 富科伊丹显示强大的抗氧化剂,抗癌,抗微生物和抗凝固特性.
- 这些发现支持富可伊丹在制药和生物医学应用中的潜力.
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