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在DENV病变发生过程中揭开tRNA片段:从RNA测序的洞察力

Deeksha Madhry¹, Kiran Kumari¹, Varsha Meena¹

¹Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India.

Scientific reports

|August 7, 2024

概括

登革热病毒 (DENV) 感染增加了tRNA碎片化,改变了tRNA衍生小RNA (tsRNA) 配置文件. 具体来说,3'tRFs被上调和i-tRFs被下调,影响宿主通路.

科学领域:

分子生物学分子生物学
病毒学病毒学
在RNA生物学,RNA生物学.

背景情况:

小型非编码RNAs (sncRNAs) 包括tRNA衍生小RNAs (tsRNAs),分类为tRNA衍生碎片 (tRFs) 和tRNA半个 (tiRNAs).
tsRNAs与人类疾病机制有关,但它们在登革热病毒 (DENV) 感染中的作用仍然未被描述.

研究的目的:

在DENV感染期间调查tsRNAs的生成和表达配置文件.
确定DENV改变的特定tsRNA群体,并分析它们对病毒病原发生的潜在影响.

主要方法:

小RNA测序被用来对DENV感染的Huh7细胞中的tsRNA进行分析.
进行了生物信息分析,包括基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.

主要成果:

DENV感染导致了tRNA分裂的增加.
鉴定出了大量差异表达的tsRNAs,其中显著的是3'tRFs的上调和i-tRFs的下调.
途径分析表明,这些tsRNAs参与了转录调节和代谢途径.

结论:

DENV感染显著改变了tsRNA表达特征.
差异表达的tsRNAs,特别是3'tRFs和i-tRFs,可能通过调节宿主转录和代谢过程,对DENV病变产生贡献.
这项研究为tsRNAs在宿主对DENV感染的反应中的作用提供了新的见解.

关键词:

这就是DENV的意义.宿主病毒的相互作用.没有编码的RNA.对于RNases来说,这是一个很好的方法.小RNA测序小RNA测序是一个很好的方法.由tRNA衍生的RNA片段

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