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一种氨酸染料的光声学成像,针对细菌感染
Rowan Swann1, Daniel Hernández-Valdés1, Luis Rafael Silva1
1Department of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, L8S 4L8, Canada.
一种使用细菌向染料的新型光声成像探针 (PIP) 在小鼠中区分了细菌感染与无菌炎症. 这一进步可以改善早期感染检测和打击抗生素耐药性.
科学领域:
- 医疗成像医学成像
- 生物技术是生物技术.
- 传染病诊断 传染病诊断 传染病诊断
背景情况:
- 非侵入性,特定于感染的诊断探针对于早期检测和向治疗至关重要.
- 降低抗生素耐药性需要改进的方法来区分感染和无菌炎症.
研究的目的:
- 评估一种由二烯胺 (ZnDPA) 衍生的光体,PSVue794,作为用于感染检测的光声成像探针 (PIP).
- 评估探针区分细菌感染与无菌炎症和健康组织的能力.
主要方法:
- 开发了一个放射性标记版本,[99mTc]Tc-PSVue794,用于定量生物分布研究.
- 利用光声学 (PA) 成像来评估细菌感染,无菌炎症和健康组织的小鼠模型中的探针.
- 在感染部位,无菌炎症和背景水平的量化PA信号.
主要成果:
- 放射标记的探头在注射后12小时显示了高的目标与非目标比率 (10.1 ± 1.1).
- 皮肤膜成像显示,在细菌感染部位 (0.176 ± 0.011 a.u.) 的信号显著更高. 与背景相比 (0.081 ± 0.04 a.u.,P ≤ 0.03) 的时间.
- 来自无菌炎症的PA信号 (每小时0.062±0.012) 在统计学上与背景没有差异 (0.058 ± 0.006 a.u. ) 的情况.
结论:
- 已建立的光体可以重新用于光声成像 (PAI).
- PAI可以有效地区分细菌感染与无菌炎症.
- 这种方法对非侵入性感染诊断和管理具有前景.
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