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Updated: Jun 17, 2025

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

宫癌患者的循环瘤细胞接受化学辐射疗法与手臂疗法相结合

Klaudia Reginacova¹, Eliska Pospisilova², Martina Kubecova¹

¹Department of Oncology, The Third Faculty of Medicine, Charles University Prague and Faculty Hospital Kralovske Vinohrady Srobarova 50, 100 34 Prague 10, Czech Republic.

American journal of cancer research

|August 8, 2024

概括

循环瘤细胞 (CTC) 可以帮助监测宫癌 (CC) 治疗. 在CC患者中增加的CTC和干细胞标志物表达可能会在标准成像之前预测复发.

科学领域:

在瘤学瘤学.
癌症生物标志物癌症生物标志物
分子诊断学分子诊断

背景情况:

循环瘤细胞 (CTC) 在监测固体瘤治疗反应方面表现有前途.
宫癌 (CC) 管理受益于可靠的预后和预测标记.

研究的目的:

调查激进化学放射治疗 (CRT) 和CC患者随访期间CTCs的发生和预测价值.
将CTC水平与SCC-Ag作为治疗监测和复发预测的生物标志物进行比较.

主要方法:

对30名接受激进CRT的CC患者进行前性研究.
使用MetaCell®和SCC-Ag测量进行序列CTC计数.
在CTC中检测干细胞标记物表达 (SOX2,POUSF) 的定量PCR (qPCR).

主要成果:

在诊断时96%的患者和治疗后61%的患者中检测到CTC.
治疗前增加的CTC和SCC-Ag与复发相关.
在临床复发检测前大约12周,CTCs在3/4的患者中增加.
与复发相关的CTC中SOX2和POUSF表达率升高 (P < 0.02).

结论:

在CC中,CTC对于评估瘤负担和疾病侵略性是有价值的.
升的CTC数量在SCC-Ag升高和成像确认的复发之前发生.
在CTC中干细胞标志物表达可能表明疾病的攻击性并预测复发.

关键词:

在CRT中,CRT可以在CRT中使用.宫癌是发生在宫癌的原因之一.在SCC中,SCC是SCC,而SCC则是SCC.支臂疗法 (brachytherapy) 是一种治疗方法.化学疗法或放射治疗循环中的瘤细胞.树干性树干性

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