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N6 - - 甲基氨酸甲基转移酶METTL14与类风湿性关节炎中的巨细胞两极分化有关

Ziheng Zhu¹, Lei Wan^1,2

¹Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui 230031, P.R. China.

Experimental and therapeutic medicine

|August 8, 2024

概括

甲基转移酶14 (METTL14) 在类风湿性关节炎 (RA) 患者中升高,与疾病严重程度和炎症相关. 通过MAPK通路促进巨细胞极化,METTL14可能会使RA恶化.

科学领域:

免疫学免疫学免疫学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

类风湿性关节炎 (RA) 的发病包括由巨细胞两极分化驱动的炎症反应.
N6-甲基氨酸 (m6A) RNA甲基化是一种影响巨细胞两极分化的表观遗传修饰.
对于m6A甲基化在RA巨分化中的确切作用尚不清楚.

研究的目的:

研究m6A甲基化,特别是甲基转移酶14 (METTL14) 的功能和机制,在RA的背景下研究巨细胞极化.
确定METTL14表达与RA临床和炎症标志物之间的相关性.

主要方法:

反转录定量PCR (RT-qPCR) 来评估m6A甲基酶基因和信号通路组件的mRNA表达.
对METTL14蛋白水平的西部斑点分析.
针对细胞分泌因子和巨细胞标记物的ELISA和流细胞计 (CD68+,CD86+).
基因和基因组的京都百科全书 (KEGG) 途径分析.

主要成果:

在RA患者的外周血液和突组织中观察到METTL14表达的升高.
METTL14水平与C反应蛋白,类风湿因子和促炎性细胞因子TNF-α正相关.
METTL14表达与M1巨标记物 (CD68+,CD86+) 相关,并预测了视觉模拟尺度得分.
METTL14与MAPK信号通路有关,在高METTL14组中增加了JNK和ERK2表达.

结论:

在RA中,METTL14是上调调的,与疾病严重程度和炎症标志物有关.
通过MAPK信号通路促进巨细胞极化,METTL14可能会加剧RA.
METTL14代表了管理RA相关炎症的潜在治疗标.

关键词:

通过MAPK路径.生物信息学分析分析巨细胞的两极分化甲基转移酶 14 的作用.

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