通过使用来自皮废物的进化适应性重组大肠杆菌 (Escherichia coli) 来实现烯的可持续生物合成
Muhammad Hammad Hussain1, Subra Sajid2, Maria Martuscelli3
1State Key Laboratory of Bioreactor Engineering East China University of Science and Technology, Shanghai, 200237, PR China.
Heliyon
|August 8, 2024
概括
皮废弃物水解剂提供可持续的,低成本的碳来源,用于生产烯. 这项研究使用工程化大肠杆菌优化了发酵条件,证明了食品和制药应用的潜力.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 可持续化学 可持续化学
背景情况:
- 果作物残留物,如皮废物 (OPW) 构成一个重大处置挑战.
- 将OPW价值化为可贵的化合物,如利科,符合循环经济原则.
- 工程微生物菌株为生物生产提供平台,但需要优化的低成本原料.
研究的目的:
- 评估皮水解剂 (OPH) 作为微生物烯生产的成本效益高的碳来源.
- 为了提高工艺化大肠杆菌菌株中莱科生物合成的效率.
- 优化发酵参数,以最大限度地提高OPH的利科产量.
主要方法:
- 稀释酸预处理和OPW的酶性水解以产生可发酵糖.
- 一种工程化大肠杆菌菌株的适应性实验室进化 (ALE),用于在d-酸上增强生长.
- 响应表面方法 (RSM) 以优化发酵介质组成 (碳源,源,温度).
- 在5L批量发酵器中发酵,使用动力建模 (Logistic和Luedeking-Piret方程).
- 生物合成的利科的定性验证和抗氧化能力评估 (植物酸,DPPH基因清除).
主要成果:
- 从OPW预处理和水解成功生产了62.18g/L的总糖.
- 在ALE治疗4个月后,工程化大肠杆菌菌株的生长率得到改善.
- 优化的发酵条件 (5.53%OPH,6.57g/L,30°C) 显著提高了烯的产量.
- 证明了 1043 mg/L 的柳科产量,增速常数为 0.441 h−1.1.
- 来自OPH的百合素表现出显著的抗氧化活性,相当于植物酸和DPPH激素清除.
结论:
- 皮水解剂是微生物烯生产的可行和经济的原料.
- 这种经过工程改造的大肠杆菌菌株,经过调整和优化,可以有效地从OPH中合成甘.
- 这项研究强调了利用水果废弃物的潜力,以可持续地生产食品和制药行业的高价值化合物.
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