在一个封闭的生物反应器中开发NK和CAR-NK扩张的可扩展过程
Xuening Wang1, Maeve Elizabeth Byrne1, Chang Liu1
1Department of Pathology, Immunology and Laboratory Medicine, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, NJ, United States.
Frontiers in immunology
|August 8, 2024
概括
这项研究提出了一种可扩展的方法,使用G-Rex 100M生物反应器产生大量的功能性自然杀手 (NK) 和仿真抗原受体NK (CAR-NK) 细胞. 这一进展支持NK细胞免疫疗法的临床应用.
科学领域:
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
- 细胞疗法细胞疗法
背景情况:
- 扩大功能性自然杀手 (NK) 和仿真抗原受体NK (CAR-NK) 细胞的生产是基于NK的免疫疗法面临的重大挑战.
- 现有的方法可能不足以实现广泛临床使用所需的大规模制造.
研究的目的:
- 开发和验证生产NK和CAR-NK细胞的大规模,可靠和实用的方法.
- 评估使用新型生物反应器系统生产的NK和CAR-NK细胞的疗效和特征.
主要方法:
- 使用G-Rex 100M生物反应器,采用气体透膜技术,可增强氧气输送和大容量培养.
- 在G-Rex 100M生物反应器中扩大了外周血液NK细胞 (PBNKs) 和CAR-NK细胞.
- 将G-Rex 100M中扩展的细胞的免疫类型和细胞毒性活性与G-Rex 6井生物反应器中的细胞进行了比较.
- 评估了冷保存对扩大NK细胞细胞毒性的影响.
主要成果:
- G-Rex 100M生物反应器系统促进了PBNK和CAR-NK的大规模扩张.
- 在G-Rex 100M生物反应器中扩展的NK和CAR-NK细胞与较小的生物反应器中的细胞相比,对肝细胞癌 (HCC) 细胞保持了类似的免疫表型和细胞毒性活性.
- 冷保存对使用这种扩大规模的方法产生的NK细胞的细胞毒性影响很小.
结论:
- G-Rex 100M生物反应器系统为扩大功能NK和CAR-NK细胞的生产提供了一个强大的平台.
- 这种可扩展的方法对开发"现成"NK细胞产品充满希望.
- 这些发现支持NK细胞免疫疗法的未来临床实施,通过增强细胞制造能力.
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