镜子辅助光片显微镜:一个简单的升级,以实现双向样本激发
Asaph Zylbertal1, Isaac H Bianco1
1University College London, Department of Neuroscience, Physiology & Pharmacology, London, United Kingdom.
Neurophotonics
|August 8, 2024
概括
镜子辅助光片显微镜 (mLSM) 克服了生物样本中的组织封闭. 这种具有成本效益的升级使以前无法访问的区域能够得到均的激发和细胞分辨率成像,从而增强光片显微镜的实用性.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 显微镜技术 显微镜技术
- 神经科学是一个神经科学.
背景情况:
- 光板显微镜提供光学切割,但与不透明的组织作斗争.
- 光片显微镜中的单向照明可以导致由于遮蔽导致的样本激发不均,如幼虫斑马鱼大脑成像中所见.
研究的目的:
- 引入镜子辅助光片显微镜 (mLSM) 作为一种改善样品照明的方法.
- 为了克服光片显微镜中单向照明的局限性,用于成像封闭的生物结构.
主要方法:
- 通过将镜像式微镜纳入现有的光板设置,开发了mLSM.
- 利用一个扫描的激光束反射出镜来创建第二个激发路径.
- 采用在线调整扫描和激光功率以实现均的双重照明.
主要成果:
- mLSM成功地对斑马鱼大脑区域进行了成像,以前由于闭塞而无法访问.
- 通过直接和反射光片照明的区域实现了细胞分辨率成像.
- 通过转换激发目标来证明可调节的成像质量.
结论:
- mLSM是光片显微镜系统的简单,廉价和有效升级.
- 这种技术通过提高激发均性来增强生物样本的数据采集.
- mLSM可以更全面地对复杂的生物结构进行成像,而不会干扰样品的行为.
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