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改进使用双点双标记水方法测量每日总能耗的质量控制程序

Matthew J Breit¹, Nassia M Duncan¹, Jared H Dahle²

¹Department of Medicine, Division of Endocrinology, Metabolism, and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.

Rapid communications in mass spectrometry : RCM

|August 8, 2024

概括

在双标记水 (DLW) 方法中扩大 (2H) 的质量控制 (QC) 范围,可以获得类似的每日总能耗 (TDEE) 结果. 这种调整可以帮助DLW实验室优化质量控制标准并降低分析成本.

科学领域:

代谢研究的研究.
同位素追踪技术是同位素的追踪技术.
人类生理学人类生理学

背景情况:

双标记水 (DLW) 方法的精度依赖于精确的同位素测量.
当前的质量控制 (QC) 程序可能需要额外的测量,当样品重复超过仪器精度时.
样本分析中的变量会影响每日总能耗 (TDEE) 计算的可靠性.

研究的目的:

通过使用双点抽样DLW方法调查扩大QC范围对TDEE计算的影响.
为了确定同位素测量的更广泛的QC接受标准是否会影响TDEE的准确性.
通过优化质量控制参数来评估DLW分析中的潜在成本节约.

主要方法:

选了来自121名个体的DLW数据,并进行了多次样本分析.
应用了更广泛的QC范围,用于二 (2H) 和氧-18 (18O) 的重复测量.
使用调整后的QC标准重新计算了TDEE,并将结果与原始计算进行了比较.

主要成果:

将2H QC范围扩展到±10.0 δ (第1天) 和±5.0 δ (最后一天) 产生与原始计算相比几乎相同的平均TDEE (2684 ± 508与2687 ± 512 kcal/day).
在使用扩大和原始QC范围计算的TDEE之间观察到强烈的相关性 (r2 = 0.97,p < 0.001).
这些发现表明,调整后的QC范围不会显著改变TDEE平均结果.

结论:

在DLW分析中扩大早期和晚期样本的2H QC范围提供了可比的TDEE平均结果.
这些发现表明DLW实验室可以优化质量控制标准,从而降低分析成本.
该研究支持在TDEE确定中使用更广泛的QC接受限值来测量.

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