在状动物的义务与自愿肌肉多核形成的基础上,Cis调节逻辑发生了变化
Christopher J Johnson1, Zheng Zhang2,3, Haifeng Zhang3
1School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA.
概括
Myomaker (Mymk) 基因促进体的差异解释了脊椎动物和带动物之间不同的肌肉发育模式. 连衣Mymk需要MyoD和Ebf转录因子,与脊椎动物不同.
科学领域:
- 进化发育生物学 进化发育生物学
- 进行比较的基因组学.
- 肌肉的发展 肌肉的发展
背景情况:
- 肌肉制造者 (Mymk) 是一个关键的蛋白质,在脊椎动物和长动物中驱动肌肉细胞融合和多核化.
- 脊椎动物的骨肌肉普遍表达Mymk,导致多核细胞.
- 长袍Mymk表达仅限于特定的多核肌肉,在单核幼虫肌肉中不存在.
研究的目的:
- 为了研究基因表达模式差异的Cis调节机制的Mymk基因表达模式在衣动物和脊椎动物.
- 为了确定控制Mymk激活的转录因子,在突尼卡特后变形肌肉中.
主要方法:
- 在衣动物和脊椎动物之间对Mymk促进器区域的比较分析.
- 在Mymk促进体内识别转录因子结合位.
- 关于Mymk调节的功能研究在西奥纳.
主要成果:
- 在Mymk促进体内的cis调节序列的差异决定了时空表达模式.
- 脊椎动物的Mymk转录仅由MyoD进行调节.
- 状Mymk转录需要MyoD和早期B细胞因子 (Ebf) 的联合作用,与促进体中的特定Ebf结合位点相关.
结论:
- 在Mymk调节中的宏观进化差异被编码在cis调节元素中.
- 肌肉发育的进化涉及转录因子结合和基因调节的变化.
- 关于Mymk和肌肉细胞融合调节的拟议模型在衣动物和脊椎动物的最后一个共同祖先中.
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