相关实验视频
Updated: Jun 17, 2025

Introduction to Solid Supported Membrane Based Electrophysiology
Published on: May 11, 2013
通过支液体膜进行空心纤维在注射器平衡采样,以评估药物与血的结合
Thaer Barri1, Ruba Ramzi2, Nasir M Idkaidek2
1Department of Chemistry, Faculty of Arts & Sciences, University of Petra, P. O. Box 961343, Queen Alia Airport Street, Amman, 11196, Jordan.
本研究介绍了一种简单的方法,使用通过支液体膜 (HFiS ESTSLM) 和RP-HPLC进行中空纤维注射器平衡采样来测量药物与血的结合. 该技术有效地确定了基本药物的药物血结合,显示了度依赖.
科学领域:
- 药理动力学和药物新陈代谢
- 分析化学 分析化学
- 生物物理化学 生物物理化学
背景情况:
- 准确确定药物与血结合 (DPB) 对于了解药物处置和疗效至关重要.
- 传统的DPB评估方法可能是复杂和耗时的.
- 开发简单,具有成本效益和敏感的DPB评估方法至关重要.
研究的目的:
- 评估通过支液体膜 (HFiS ESTSLM) 通过空心纤维在注射器平衡采样以及RP-HPLC来确定药物与血结合的疗效.
- 评估具有不同物理化学性质的三个模型弱基药的DPB.
主要方法:
- 通过支液体膜 (HFiS ESTSLM) 采用中空纤维在注射器平衡采样作为主要采样技术.
- 逆相高性能液体染色学 (RP-HPLC) 用于药物的定量分析.
- 该方法应用于三种模型药物:美托普罗罗尔,二胺和西尔德纳菲尔.
主要成果:
- HFiS ESTSLM 方法在检测模型药物中依赖药物的 DPB 中表现出灵敏度.
- 药物与血的结合量随着药物的水友性增加而增加,并且依赖于度,特别是在更高度时.
- 结果证实了HFiS ESTSLM技术在DPB评估中的可靠性.
结论:
- 结合的HFiS ESTSLM和RP-HPLC方法提供了一种简单,高效和具有成本效益的程序来评估药物与血的结合.
- 该方法适用于评估具有不同性质的基本药物的DPB.
- 这些发现支持HFiS ESTSLM作为药理动力学研究中宝贵的工具的实用性.
更多相关视频
09:35Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
Published on: November 29, 2014
07:55Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
Published on: June 21, 2019
相关概念视频
Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
Drug Distribution: Plasma Protein Binding
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Physiological Pharmacokinetic Models: Assumption with Protein Binding
Hepatic Drug Clearance: Effect of Protein Binding
For low-extraction-ratio drugs that are less than 80% protein-bound, minor changes in protein binding...
Drug Concentrations: Measurements
Plasma...