来自儿童COVID-19患者的血细胞外囊泡调节基于疾病严重性的单细胞和T细胞免疫反应
Pınar Gur Cetinkaya1, Irem Fatma Abras1, Irem Evcili1
1Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.
Immunological investigations
|August 8, 2024
概括
来自严重COVID-19患者的细胞外囊泡 (EV) 抑制了健康细胞中的免疫反应. 这些血衍生的EV可能在严重的儿科COVID-19期间在早期免疫抑制中发挥作用.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 随着COVID-19的流行,全球的发病率和死亡率大幅上升.
- 儿科COVID-19患者中血衍生的细胞外囊泡 (EVs) 的具体作用尚不清楚.
研究的目的:
- 为了研究小儿COVID-19患者血衍生的EVs对免疫细胞功能的影响.
- 探索COVID-19严重程度与EVs介导的免疫反应之间的关系.
主要方法:
- 细胞外囊泡 (EVs) 从健康对照组和儿童COVID-19患者中分离出来,疾病严重程度各不相同.
- 实验室试验,流细胞计和ELISA用于评估EV对健康的外周血液单核细胞 (PBMC),原始CD4+T细胞和单细胞的影响.
主要成果:
- 严重的急性COVID-19病例显示细胞因子增加,IFNγ减少,CD4+T细胞和单细胞数量降低,表明免疫抑制.
- 来自严重急性患者的EV刺激健康细胞增加PD-L1表达,促进Th2和Treg细胞,减少IFNγ,并改变Th1/Th17比率.
- 在受刺激的健康PBMC中,患者衍生的EV显著降低了单细胞的益炎性细胞因子产生,并减少了CD4+T细胞和单细胞群.
结论:
- COVID-19的严重程度与明显的免疫反应和EV介导的影响相关.
- 血衍生的EV可能有助于重症COVID-19患者的早期免疫抑制.
关键词:
在CD4+T细胞中.在 COVID-19 疫情中,这就是SARS-CoV-2病毒.细胞外囊泡 (EVs) 的产生.免疫抑制可以抑制免疫力.单细胞 (Monocytes) 是一种单细胞.编程细胞死亡1联体 (PDL1)更多相关视频
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