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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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High-Performance Liquid Chromatography: Types of Detectors01:15

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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相关实验视频

Updated: May 6, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
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在食品中检测多谱病原体,使用多重超分支的盐状滚动圆放大器.

Nanfack V D Carole1, Lina Sheng1, Jian Ji1

  • 1School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu, 214122, PR China.

Talanta
|August 8, 2024
PubMed
概括

一种新的三次探针复合滚动圆放大方法可以快速准确地检测沙门氏菌Typhimurium和黄金葡萄球菌. 这项技术为食品传播病原体检测提供了简单的视觉识别.

关键词:
食品安全 食品安全异热核酸放大效应 异热核酸放大效应病原体检测检测 病原体检测滚动圈放大器 滚动圈放大器

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Visual Detection of Multiple Nucleic Acids in a Capillary Array
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Visual Detection of Multiple Nucleic Acids in a Capillary Array

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相关实验视频

Last Updated: May 6, 2026

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Visual Detection of Multiple Nucleic Acids in a Capillary Array
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科学领域:

  • 食品安全 食品安全
  • 微生物学 微生物学
  • 分子诊断学 分子诊断学

背景情况:

  • 由沙门氏菌和黄金葡萄球菌引起的食物传播疾病对公众健康构成重大风险.
  • 目前的检测方法可能是复杂和耗时的.
  • 需要简单,快速,准确的细菌检测技术.

研究的目的:

  • 开发一种简单的方法,同时检测和识别沙门氏菌Typhimurium和金黄色葡萄球菌.
  • 为了提高灵敏度和特异性,使用三次探针复合滚动圆放大技术.

主要方法:

  • 一种三探多重滚动圆放大试验被设计为针对特定基因 (invA为S. Typhimurium, glnA为S. aureus).
  • 用光标记的探头和基于pH的检测方法被用于视觉识别.
  • 该测试以速度和灵敏度优化,用合成标和真实细菌进行测试,并在牛奶样本中进行评估.

主要成果:

  • 该试验可以在30分钟内对合成标和2小时内对真实细菌同时检测出S. Typhimurium和S. aureus.
  • 观察到高特异性,检测极限在牛奶样本中低至10 CFU/mL (S. Typhimurium) 和5 CFU/mL (S. aureus).
  • 检测方法显示稳定超过3个月.

结论:

  • 开发的三次探针复合滚动圆放大方法是特定的,敏感的,易于解释的.
  • 这种技术为在食品样本中快速可靠地检测食源性病原体提供了有希望的解决方案.
  • 该方法有助于提高食品安全,减轻食源性疾病的影响.