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在体外测试Aerococcus urinae的敏感性测试中评估梯度扩散

Kendall Kling¹, Adil Menon², Paige Norconk²

¹Department of Pathology, Northwestern University Feinberg School of Medicine, 303 E. Chicago Ave., Ward 3140, Chicago IL, 60611, USA; Department of Medicine, Division of Infectious Diseases, Northwestern University Feinberg School of Medicine, 676 N. St Clair Street, Suite 940, Chicago, IL, 60611, USA.

Journal of global antimicrobial resistance

|August 8, 2024

概括

对Aerococcus urinae的渐变扩散测试表明检测耐药菌株的局限性. 建议在渐变扩散上进行汁微稀释,以进行准确的抗微生物敏感性测试.

科学领域:

临床微生物学临床微生物学
抗微生物耐药性抗微生物耐药性

背景情况:

对Aerococcus urinae的抗微生物敏感性测试对于有效治疗至关重要.
微稀释 (BMD) 与溶解马血 (LHB) 和梯度扩散 (GD) 是常见的方法.
将这些方法进行比较对于准确的诊断至关重要.

研究的目的:

为了比较GD与BMD的准确性,用于A. urinae抗菌感应性测试.
为了评估GD的性能,使用Mueller-Hinton合剂补充羊血.

主要方法:

190个A. urinae分离物被对16种抗微生物药物进行了测试.
用穆勒-欣顿汁+LHB进行汁微稀释,与用穆勒-欣顿 agar+羊血进行GD进行比较.
用LHB稀释的阿加尔被用于trimethoprim-sulfamethoxazole的验证.

主要成果:

没有一种抗微生物药物显示了BMD和GD之间>90%的基本和分类一致.
三甲-硫甲醇与GD有81%的重大错误率.
塞夫特里亚克松与GD的错误率为76%非常严重.

结论:

由于检测耐药菌株的局限性,不建议对A. urinae进行羊血的渐变扩散.
A. urinae通常表现出对青素,线化物,四环素和万科迈的敏感性.
对替代敏感性测试方法的进一步研究是有必要的.

关键词:

这种病原体是Aerococcus urinae.抗微生物敏感性测试试验抗微生物敏感性测试调料稀释剂调料稀释剂渐变扩散的渐变扩散.验证验证验证验证

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