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相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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相关实验视频

Updated: Jun 17, 2025

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
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智能并行自动化冷电子断层扫描仪

Fabian Eisenstein1,2, Yoshiyuki Fukuda3, Radostin Danev4

  • 1Graduate School of Medicine, University of Tokyo, Tokyo, Japan.

Nature methods
|August 8, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了SPACEtomo,这是一种机器学习工作流程,可以完全自动化冷电子断层扫描. 这种自动化是细胞中高分辨率宏分子结构确定的关键.

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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
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相关实验视频

Last Updated: Jun 17, 2025

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11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

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科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学

背景情况:

  • 在现场冷电子断层扫描 (cryo-ET) 允许在它们的原生细胞环境中可视化宏分子.
  • 最近硬件和软件的进步增加了冷ET的样本可访问性.
  • 目前的冷ET数据收集是劳动密集型的,需要专家操作人员和显著的显微镜时间来选择目标和倾斜系列采集.

研究的目的:

  • 为冷电子断层扫描开发一个完全自动化的工作流程.
  • 消除在冷ET数据收集中需要人类干预的需要.
  • 为了实现结构分析的统计相关数据集的高通量获取.

主要方法:

  • 开发了SPACEtomo,这是一个集成机器学习的新型工作流.
  • 实现了自动化斑块检测和生物特征细分.
  • 启用了自动目标选择和并行倾斜系列采集.

主要成果:

  • SPACEtomo完全自动化了整个冷电子断层扫描过程.
  • 工作流程不需要人类干预.
  • 促进结构研究的高通量数据收集.

结论:

  • SPACEtomo显著推进了冷电子断层扫描的自动化.
  • 这种自动化对于获得统计相关数据集至关重要.
  • 能够在它们的原生细胞环境中对宏分子的高分辨率结构进行确定.