免疫遗传学多形态和表达对慢性C型肝炎中直接作用抗病毒药物反应的影响
Aya Ismail Abdelaziz1, Eman Abdelsameea2, Mohamed Abdel-Samiee2
1Department of Research and Development, Faculty of Pharmacy, Heliopolis University for Sustainable Development, Cairo, Egypt.
Clinical and experimental medicine
|August 8, 2024
概括
主体遗传因素,特别是IL-28B和FOXP3基因多态性,在埃及患者中显著影响C型肝炎病毒 (HCV) 对直接作用抗病毒药物的治疗反应. 较低的IL-28B和较高的FOXP3水平与治疗结果不佳相关.
科学领域:
- 免疫遗传学 免疫遗传学
- 肝病学 肝病学是一种肝病学.
- 药物基因组学 药物基因组学
背景情况:
- 埃及的C型肝炎病毒 (HCV) 感染率在直接作用抗病毒 (DAA) 治疗下降.
- 对DAA的治疗反应受到宿主免疫遗传的影响,包括Interleukin-28B (IL-28B) 和Forkhead盒子P3 (FOXP3) 多态.
研究的目的:
- 调查FOXP3基因促进区和IL28B基因中单核酸多态 (SNP) 对埃及患者HCV治疗反应的影响.
- 评估IL-28B和FOXP3的血清水平和DAA治疗结果之间的关联.
主要方法:
- 通过实时PCR和RFLP-PCR进行IL28B (rs12979860,rs8099917) 和FOXP3 (rs3761548,rs2232365) SNPs的基因组化.
- 通过ELISA量化血清IL-28B和FOXP3水平.
- 对99名获得持续病毒反应 (SVR12) 的患者和63名治疗失败的患者的治疗反应的分析.
主要成果:
- IL28B rs12979860 T>C和FOXP3 rs2232365 A>G多态性与非响应风险的增加显著相关 (分别P=0.013和P=0.008).
- 与没有反应者相比,响应者表现出较高的血清IL-28B水平 (P=0.046) 和较低的FOXP3水平 (P<0.001).
- 一个结合IL28B rs12979860和FOXP3 rs2232365的预测模型显示,DAA反应的敏感度为76.2%,特异性为91.9%.
结论:
- IL28B rs12979860 T>C和FOXP3 rs2232365 A>G多态性显著影响埃及HCV患者的DAA治疗反应.
- 较低的IL-28B和较高的FOXP3血清水平与治疗结果不佳有关.
- 这些发现有助于个性化医疗,改善HCV患者的治疗决策.
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