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长非编码RNANKX2-1-AS1通过miR-589-5p/NME1轴加速非小细胞肺癌的进展

Xiaoying Chen¹, Ruilai Jiang¹, Xiaocheng Huang¹

¹Department of Respiratory and Critical Care Medicine, The Second Peoples' Hospital of Lishui, Lishui, China.

Cell biochemistry and biophysics

|August 8, 2024

概括

长非编码RNANKX2-1-AS1通过通过miR-589-5p轴向上调节NME1,促进非小细胞肺癌 (NSCLC) 的进展. 这一发现为NSCLC治疗提供了潜在的治疗点.

科学领域:

分子瘤学分子瘤学
癌症生物学癌症生物学
在RNA生物学,RNA生物学.

背景情况:

非小细胞肺癌 (NSCLC) 是全球癌症死亡的主要原因.
驱动NSCLC进展的精确机制,特别是长非编码RNAs (lncRNAs) 的作用,仍然不完全理解.
NKX2-1反感RNA 1 (NKX2-1-AS1) 被认为是肺瘤发生的一个瘤基因.

研究的目的:

阐明NKX2-1-AS1在NSCLC进展中的潜在机制.
研究NKX2-1-AS1在NSCLC细胞行为和瘤生长中的调节作用.

主要方法:

定量逆转录PCR (RT-qPCR) 用于基因表达分析.
细胞增殖试验 (MTT,EDU),细胞亡分析 (流细胞计),迁移和入侵试验 (Transwell).
西方斑点,免疫光,RNA下拉,光酶记者测定和异种移植小鼠模型.

主要成果:

在NSCLC组织和细胞系中,NKX2-1-AS1表达显著上调.
NKX2-1-AS1缺乏抑制了细胞的增殖,迁移,入侵和上皮细胞-介质细胞过渡 (EMT),同时促进了细胞亡.
NKX2-1-AS1与miR-589-5p直接相互作用,miR-589-5p针对NME/NM23核酸二酸酶1 (NME1),从而调节miR-589-5p/NME1轴,并在体内抑制瘤生长.

结论:

NKX2-1-AS1通过通过miR-589-5p/NME1轴对NME1进行上调来促进NSCLC的进展.
NKX2-1-AS1在NSCLC中起着至关重要的致癌因素的作用.
NKX2-1-AS1/miR-589-5p/NME1通路代表了NSCLC的一个潜在的治疗点.

关键词:

皮质-介质细胞过渡.在LncRNA NKX2-1-AS1中,在米R-589-5p/NME1轴上非小细胞肺癌 (NSCLC) 是一种非小细胞肺癌.

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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...