在骨质疏松与类风湿性关节炎的关键基因和长非编码RNA表达特征的识别,基于生物信息学分析
Jin-Yu An1, Xing-Na Ma2, Hui-Long Wen3
1Department of Orthopedics, Changzhou Fourth People's Hospital, Changzhou, 213000, China. ajy_428@126.com.
BMC musculoskeletal disorders
|August 8, 2024
概括
这项研究确定了类风湿性关节炎 (RA) 和骨质疏松症 (OP) 的共同分子途径和基因,揭示了这些相互关联的疾病的潜在治疗点. 研究结果强调RP11-815J21.2,MT-ATP6和PTGS1是RA和OP病变发生的关键因素.
科学领域:
- 基因组学和生物信息学
- 分子生物学分子生物学
- 类风湿病学 类风湿病学
- 内分泌学 在内分泌学.
背景情况:
- 类风湿性关节炎 (RA) 是一种慢性系统性疾病,经常与骨质疏松症 (OP) 一起发生.
- 连接RA和OP的潜在分子机制在很大程度上是未知的.
- 这项研究使用生物信息学研究共享的分子病原体.
研究的目的:
- 识别RA和OP共同的差异表达基因 (DEGs) 和长非编码RNA (lncRNAs).
- 阐明与这些共享的DEGs相关的分子途径和生物功能.
- 构建一个编码-非编码网络,并确定潜在的治疗点.
主要方法:
- 从 RA 和 OP 患者的 Gene Expression Omnibus (GEO) 数据库中检索了基因表达特征.
- 进行差异表达分析,基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
- 构建的蛋白质-蛋白质相互作用 (PPI) 和编码-非编码网络,随后使用qRT-PCR和基于细胞的测试进行实验验证.
主要成果:
- 鉴定了28个差异表达的mRNA (DEmRNA) 和2个与RA和OP共同的DElncRNA.
- 丰富性分析显示了与血小板脱粒,激活和白细胞透内皮移动的关联.
- 确定MT-ATP6和PTGS1作为潜在的枢纽基因,RP11-815J21.2作为关键的lncRNA,实验验证证证了它们的差异表达和RP11-815J21.2在骨质细胞形成中的作用.
结论:
- 在RA和OP中成功确定了共享的失调基因和lncRNAs,强调了它们的分子相互联系.
- 这些发现为RA和OP的病变产生提供了新的见解.
- 建议潜在的治疗点,如RP11-815J21.2,MT-ATP6和PTGS1,用于进一步的研究和有针对性的治疗策略.
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