相关实验视频

Updated: Jun 17, 2025

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Published on: June 25, 2015

TetR家族调节器 AbrT 控制了Streptomyces lincolnensis 中的林氏素生产和形态发展

Yurong Xu^1,2, Meng Liu³, Ruidong Zhao³

¹Department of Chemical and Pharmaceutical Engineering, Hefei Normal University, Hefei, 230601, China. xuyurong89@163.com.

Microbial cell factories

|August 8, 2024

概括

在Streptomyces lincolnensis中,AbrT是一种TetR家族调节剂,控制着林氏素的产生和发育. D-arabinose作为其连接体,调节抗生素生物合成,并提供对二次代谢调节的见解.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

TetR家族调节器 (TFRs) 通过连接体诱导的构造变化来控制细胞过程.
虽然 Actinomycetes 中的 TFR 配体已知,但它们在Streptomyces lincolnensis 中的林氏素生产中的作用尚不清楚.

研究的目的:

研究Streptomyces lincolnensis中TetR家族调节器AbrT的功能.
阐明林氏素生物合成和形态发展的调节机制.

主要方法:

在S. lincolnensis中删除abrT的基因.
分析林氏素的产生和形态发展.
林群和发育基因的基因表达分析.
电泳运动转移试验 (EMSAs) 用于确定DNA结合.
AbrT效应分子的识别.

主要成果:

AbrT删除增加了林氏素A的产生,但延迟了发育.
AbrT抑制了林氏素生物合成基因并激活了发育基因.
D-arabinose被确定为AbrT的效应分子,减少其DNA结合亲和力.

结论:

在S. lincolnensis中,AbrT调节了林氏素的产生和形态的发展.
D-阿拉比诺酸通过AbRT调节林氏素生物合成的调节.
对抗生素生物合成的联体介导调节的新见解.

关键词:

这种植物是Streptomyces lincolnensis.抗生素的生物合成.D-阿拉比诺糖的使用方法连接物连接物连接物调节器是TetR家族的调节器.

更多相关视频

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Published on: November 24, 2017

相关概念视频

Prokaryotic Transcriptional Activators and Repressors

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

Operons

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...