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相关概念视频

CRISPR01:59

CRISPR

50.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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相关实验视频

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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利用不断发展的CRISPR/Cas9进行精密瘤学.

Tianye Li1,2, Shuiquan Li3, Yue Kang4

  • 1Department of Gynecology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, 310009, People's Republic of China.

Journal of translational medicine
|August 8, 2024
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概括

聚类正规间隔短时间的Palindromic重复 (CRISPR) /Cas9基因编辑系统为了解和治疗癌症等疾病提供了精确的DNA操纵. 目前正在进行的创新旨在克服其在精密瘤学中的临床应用的挑战.

关键词:
基础编辑器 基础编辑器这就是CRISPR/Cas9的作用.化学抗原受体的化学抗原受体精确瘤学是一门精确的专业.这就是SpCas9的意义.dCas9 的使用情况

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科学领域:

  • 遗传学和分子生物学
  • 生物技术是生物技术.
  • 在瘤学瘤学.

背景情况:

  • 来自微生物免疫的聚类正规间隔短时间Palindromic重复 (CRISPR) / Cas9系统,提供精确,多功能DNA编辑能力.
  • 它的应用已经超出了基础研究的范围,导致了诸如状细胞贫血的CASGEVYTM等治疗进步.

研究的目的:

  • 探索CRISPR/Cas9在理解癌症生物学中的多方面的应用,从基因功能调查到疾病建模.
  • 突出CRISPR/Cas9在开发新型个性化癌症疗法和加强CAR-T等免疫疗法的作用.
  • 讨论CRISPR/Cas9在癌症干预预防性基因查中的潜力.

主要方法:

  • 利用CRISPR/Cas9精确操纵哺乳动物系统中的基因.
  • 采用CRISPR/Cas9来建模各种癌症类型并研究瘤生物学,包括耐药性和免疫逃避.
  • 利用CRISPR/Cas9进行查易患癌症的基因,并加强向癌症治疗.

主要成果:

  • 克里斯普尔/Cas9有助于更深入地了解癌症遗传学和生物学.
  • 该系统可以开发有针对性的癌症治疗方法和改进的CAR-T疗法.
  • CRISPR/Cas9有助于识别用于早期癌症干预策略的基因.

结论:

  • 克里斯普尔/Cas9是一种变革性的工具,彻底改变了癌症研究和治疗模式.
  • 尽管存在有效性,效率和安全方面的挑战,但持续的技术创新至关重要.
  • 将CRISPR/Cas9集成到临床实践中,预示着精确瘤学的新时代,并有可能进行治愈疗法.