一种双色仪-电化学微流体基于纸张的分析装置,用于对缺血性中风的临床检测
Silvia Dortez1, Marta Pacheco2, Teresa Gasull3
1Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcala, 28802, Alcala de Henares, Madrid, Spain. alberto.escarpa@uah.es.
Lab on a chip
|August 9, 2024
概括
一种新的双传感器设备快速准确地测量了缺血性中风患者的转激素和 (TSAT). 这种临床测试为诊断中风提供了更快,更可靠的替代方案.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 转激素和 (TSAT) 是诊断缺血性中风的关键生物标志物.
- 目前的TSAT评估方法耗时且复杂,阻碍了快速的临床决策.
- 缺血性中风需要快速,准确和护理点诊断工具.
研究的目的:
- 开发一种基于纸的微流体分析装置 (μPAD),用于TSAT的同时色度和电化学检测.
- 通过使用经过认证的参考材料和来自缺血性中风患者的临床样本来验证双μPAD的性能.
- 建立μPAD作为一个可靠和有效的工具,用于在缺血性中风诊断中进行点诊断TSAT评估.
主要方法:
- 设计了一种基于纸张的微流体分析装置 (μPAD),配备了集成的色度和电化学检测储.
- 转素结合的铁被测量色度,和铁的总结合能力被测量电化学计算TSAT.
- PAD与经认证的参考材料进行了验证,并与使用缺血性中风患者血清样本的尿素-PAGE方法进行了比较.
主要成果:
- 在分析经过认证的参考材料时,双μPAD显示出卓越的准确性 (Er ≤ 5%) 和精度 (RSD ≤ 2%).
- 对缺血性中风患者样本的分析显示,与已建立的尿素-PAGE方法有很强的相关性 (r = 0.96,p < 0.05).
- PAD只需要90μL的样品和<90分钟,比18小时的尿素-PAGE方法要快得多.
结论:
- 开发的双色仪-电化学μPAD是一种有前途的无试剂设备,用于点的护理TSAT测试.
- 这项技术提供了一种快速,准确和可靠的方法,帮助医生快速诊断和预测缺血性中风.
- PAD有可能通过在时间关键的中风情况下实现更快的临床决策来改善患者的治疗结果.
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