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相关概念视频

Computed Tomography01:10

Computed Tomography

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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Imaging Studies I: CT and MRI01:14

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Introduction: MRI and CT scans are crucial advancements in medical imaging techniques, playing a vital role in diagnosing conditions related to the gastrointestinal (GI) system. Each scan serves distinct purposes, targets specific areas, and requires unique nursing duties.
Description of the Procedures
Computed Tomography (CT) scan:
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Positron Emission Tomography01:29

Positron Emission Tomography

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Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
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Brain Imaging01:14

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Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
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X-ray Imaging01:24

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German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
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相关实验视频

Updated: Jun 17, 2025

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
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Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

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阵列断层扫描:通向发现的路径

Kristina D Micheva1, Jemima J Burden2, Martina Schifferer3,4

  • 1Department of Neurosurgery, Stanford University, Stanford, CA, 94305, USA.

Methods in microscopy
|August 9, 2024
PubMed
概括
此摘要是机器生成的。

阵列断层扫描 (AT) 可使用序列切割和先进显微镜进行详细的3D生物结构分析. 新的发展简化了AT,使研究人员更容易使用这种强大的技术.

关键词:
这就是ATUM ATUM的意义.阵列断层扫描 阵列断层扫描光显微镜光学显微镜连续切割的系列切割.这是一种超微微形手术 (ultramicrotomy).卷电子显微镜的体积电子显微镜.

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相关实验视频

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科学领域:

  • 生物成像成像技术
  • 显微镜技术的使用方法
  • 结构生物学是结构生物学.

背景情况:

  • 组织切片对于研究生物结构至关重要.
  • 容量电子显微镜 (vEM) 方法,如阵列断层扫描 (AT) 提供先进的3D分析.
  • AT结合了串行超微缩切除术,截面成像和数据重组.

研究的目的:

  • 为突出生物研究的阵列断层扫描 (AT) 的进步.
  • 通过详细说明解决方案和克服约束来解决AT的不足.
  • 引导研究人员选择合适的vEM技术并探索AT的潜力.

主要方法:

  • 序列超微切割术用于超薄切割.
  • 多模式成像 (光学和扫描电子显微镜).
  • 图像重新组装和分析用于3D体积重建.

主要成果:

  • AT提供了长期样本保存和多尺度成像兼容性.
  • 序列切割增强了轴分辨率,并为相关显微镜的分子标记提供了便利.
  • 截图和图像分析的新发展简化了AT管道.

结论:

  • 阵列断层扫描为多尺度和多模式生物成像提供了显著的优势.
  • 通过新的发展和培训克服感知到的困难可以增加AT的采用.
  • 在各种生物领域中,AT具有巨大的未开发潜力,有助于结构和分子洞察力.