针对SARS-CoV-2病毒蛋白的氧化结合蛋白调节了冠状病毒的复制
Yue Ma-Lauer1,2, Pengyuan Li1,2, Daniela Niemeyer3,4
1Virology Department, Max-von-Pettenkofer Institute, Ludwig-Maximilians-University Munich, Munich, Germany.
Frontiers in cellular and infection microbiology
|August 9, 2024
概括
氧化醇结合蛋白 (OSBP) 促进了冠状病毒的复制. 一种新的化合物ZJ-1针对OSBP,降低病毒水平并显示对SARS-CoV-2和其他冠状病毒的抗病毒作用.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 氧化醇结合蛋白 (OSBP) 对于细胞器官之间的脂质运输至关重要.
- 在包括SARS-CoV-2在内的冠状病毒复制中,OSBP的作用尚不清楚.
- 了解OSBP在病毒复制中的功能对于开发新的抗病毒策略至关重要.
研究的目的:
- 调查OSBP在冠状病毒复制中的作用.
- 评估一种新型的OSBP结合化合物ZJ-1,作为对冠状病毒的潜在抗病毒剂.
- 为了阐明OSBP和SARS-CoV-2蛋白质之间的分子相互作用.
主要方法:
- 生物化学和细胞分析被用来研究OSBP相互作用.
- 评估了ZJ-1对OSBP水平和病毒复制的影响.
- 确定了特定的病毒蛋白及其与OSBP和VAP-B的相互作用.
主要成果:
- 发现OSBP可以积极调节冠状病毒复制.
- 采用ZJ-1治疗降低了OSBP水平,并对多种冠状病毒表现出强大的抗病毒活性.
- 确定了OSBP和SARS-CoV-2非结构蛋白 (Nsps) 3,4和6之间的相互作用,影响了双膜囊泡的形成.
- SARS-CoV-2 orf7a,orf7b和orf3a被确定为OSBP目标,OSBP稳定了这些蛋白质.
结论:
- OSBP在冠状病毒复制中发挥着重要作用,是潜在的治疗点.
- 结合OSBP的化合物ZJ-1显示出作为抗病毒剂对抗SARS-CoV-2和其他冠状病毒的承诺.
- 针对OSBP介导的途径提供了一种新的策略来对抗冠状病毒感染.
更多相关视频
相关概念视频
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Conjugated Proteins
18.3K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.3K
Co-activators and Co-repressors
7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
Regulation of Expression at Multiple Steps
879
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
879
Cholesterol: Significance and Regulation
524
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Considering cholesterol and...
524


