一种先进的三维现象型测量方法,用于提取根基根基因结构参数,基于地面激光扫描
Yinyin Liang1, Kai Zhou1, Lin Cao1
1Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Frontiers in plant science
|August 9, 2024
概括
一种新的方法,准确而详细的基于定量结构模型 (基于AdQSM) 的根系表型测量 (ARPM),自动化了植物根系表型. 与传统的3D模型相比,这种方法可以提高根直径 (RD) 和横根计数的测量精度.
科学领域:
- 植物科学 植物科学
- 林业林业 林业 林业 林业
- 计算生物学 计算生物学
背景情况:
- 传统的植物根表型是劳动密集型和有限的,特别是对于木质物种.
- 精确的根系表型是提高植物生产力和适应性的关键.
- 开发自动化方法对于有效的根系分析至关重要.
研究的目的:
- 开发一种基于定量结构模型 (基于QSM) 的根系表型测量 (ARPM) 的自动化方法,用于金科树根系.
- 为了比较ARPM的准确性与传统的3D重建方法 (基于MST和基于TIN).
- 为了确定提取根结构表型的最佳参数.
主要方法:
- 使用陆地激光扫描 (TLS) 点云的金科根系统的三维 (3D) 重建.
- 开发一种基于QSM (基于AdQSM) 的准确和详细的根系表型测量 (ARPM) 方法.
- 使用ARPM,基于MST和基于TIN的模型提取和比较根系表型参数 (根系直径,长度,表面积,体积,侧向根数).
主要成果:
- 从点云直接使用的ARPM产生了非常准确的根直径 (RD) 测量 (R2 = 0.99,RMSE = 0.41 cm),性能优于3D模型.
- 基于最小跨度树 (MST) 的模型显示,在横向根检测方面,基于三角形不规则网络 (TIN) 的模型 (F1 = 0.80) 的模型 (F1 = 0.81) 的性能略好 (F1 = 0.81).
- 确定0.002的云参数 (CP) 值是提取结构表型的最佳值 (r = 0.94,p < 0.01).
结论:
- 基于AdQSM的ARPM方法提供了一个更准确和自动化的方法,用于Ginkgo根表型.
- 从点云中直接提取RD优于使用3D重建模型.
- 这项研究增强了对树根结构及其与生理功能的关系的理解.
相关概念视频
Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Electron Microscope Tomography and Single-particle Reconstruction
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Three-Dimensional Microscopy in Microbiology
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...


