复杂怀孕后母亲的先天性免疫重编程
Nakeisha A Lodge-Tulloch1, Jean-François Paré1, Camille Couture2,3,4
1Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
概括
孕前 (PE) 和胎儿生长限制 (FGR) 的妇女在分娩前表现出对单细胞的免疫耐受性. 这种免疫重编程可能会将妊娠并发症与以后的心血管和代谢疾病风险联系起来.
科学领域:
- 免疫学 免疫学 免疫学
- 生殖医学 生殖医学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 孕前 (PE) 和胎儿生长限制 (FGR) 与母亲的炎症和未来心血管/代谢疾病风险的增加有关.
- 天生的免疫细胞的表观遗传重编程可能是这种增加的疾病易感性的基础.
研究的目的:
- 在分娩前研究PE和FGR妇女循环单细胞的免疫表型.
- 探索与这些妊娠并发症相关联的单细胞中的潜在表观遗传修饰.
主要方法:
- 从PE,FGR和分娩前没有并发症的怀孕妇女中分离出单细胞.
- 在脂多糖 (LPS) 刺激后测量了细胞因子释放 (IL-10,IL-1β,GM-CSF,TNFα).
- 分析了TNF促进子区域和单细胞转录基因特征中的素修饰 (H3K4me3).
主要成果:
- 来自PE/FGR女性的单细胞在LPS后表现出改变的细胞因子分泌 (增加IL-10,减少IL-1β/GM-CSF) .
- 在复杂怀孕的单细胞的TNF促进区域观察到H3K4me3水平的降低.
- 转录组分析显示,在PE中,抗炎性髓状细胞的比例较高,炎性非经典单细胞较少.
结论:
- 来自PE和FGR妇女的单细胞在分娩前表现出一种免疫耐受性表型.
- 需要进一步的研究来确定这种表型是否在分娩后持续存在,并有助于长期疾病风险.
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