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Duddingtonia flagrans及其原始蛋白质溶解提取物:在绵羊共同培养中同时作用

Debora Castro de Souza¹, Elias Honorato Gomes¹, Lisseth Bibiana Figueroa Puentes¹

¹Laboratório de Biotecnologia E Bioquímica Aplicada, Departamento de Química, Universidade Federal de Lavras, Lavras, MG, Brasil.

Veterinary research communications

|August 9, 2024

概括

细菌菌菌Duddingtonia flagrans (AC001) 和其原始提取物有效控制了绵羊便中的胃肠细菌 (GIN) 幼虫. 这为反动物寄生虫感染提供了有前途的生物控制.

科学领域:

兽医寄生虫学兽医寄生虫学
菌类学菌类学是指菌类学.
生物技术是生物技术.

背景情况:

胃肠道线虫 (GIN) 感染是反动物群中的一个重大问题,是由牧场上的感染性幼虫驱动的.
有效的控制策略对于预防复发性感染和保持群体健康至关重要.

研究的目的:

为了评估Duddingtonia flagrans (AC001) 的有效性,特别是其蛋白质分析原料提取物和虫,用于体外控制GIN L3幼虫.
评估AC001及其提取物在便环境中的GIN幼虫的直接影响.

主要方法:

来自D. flagrans (AC001) 的蛋白质化原料提取物的生产.
在体外共培养试验中,使用自然感染绵羊的便样本进行测试.
治疗组包括对照组 (变质提取物),活性提取物和与AC001 conidia.有活性提取物.
幼虫恢复和统计分析 (p < 0.01),以确定治疗疗效.

主要成果:

杜丁顿 (Duddingtonia flagrans) (AC001) 成功产生了蛋白酶,其活性为56.34U/mL.
使用活性蛋白质溶解原料提取物和提取物与虫结合的治疗,与对照组相比,显著减少了GIN L3幼虫.
AC001及其提取物在便环境中表现出对虫的同时作用.

结论:

杜丁顿 (Duddingtonia flagrans) (AC001) 和其蛋白质分析原料提取物显示出对GIN L3幼虫的生物控制具有显著的潜力.
这些发现表明,一种有希望的,环保的方法来控制反动物的寄生虫感染.
对AC001的进一步实地应用在动物群中的线虫控制是有必要的.

关键词:

生物化学控制的方法便环境便环境食菌类的真菌是有毒的真菌.蛋白质酶可以保护

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